Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,344,420 C→A 43.5% P95P (CCG→CCT wzb ← protein‑tyrosine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,344,4200CA43.5% 52.7 / 97.1 101P95P (CCG→CCTwzbprotein‑tyrosine phosphatase
Reads supporting (aligned to +/- strand):  ref base C (11/46);  new base A (22/22);  total (33/68)
Fisher's exact test for biased strand distribution p-value = 1.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA  >  minE/1344353‑1344479
                                                                   |                                                           
gATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                             <  1:1152519/68‑1 (MQ=255)
gATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                             <  1:2385612/68‑1 (MQ=255)
 aTACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                             <  1:2087488/67‑1 (MQ=255)
                    caTTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                         <  1:1445926/68‑1 (MQ=255)
                    caTTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                         <  1:1554264/68‑1 (MQ=255)
                    caTTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                         <  1:1886247/68‑1 (MQ=255)
                    caTTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                         <  1:2728514/68‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTcta                                    <  1:2776469/69‑3 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:3345696/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2098435/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2788875/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1781424/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1007533/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1887940/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2366757/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2362377/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1986372/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2255819/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:97795/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1400570/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:982915/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:799431/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:890074/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:838841/69‑1 (MQ=255)
                        cGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1336424/69‑1 (MQ=255)
                          ttATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                   <  1:2109968/68‑1 (MQ=255)
                          ttATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                   <  1:952737/68‑1 (MQ=255)
                          ttATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                   <  1:1189984/68‑1 (MQ=255)
                          ttATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                   <  1:3395620/68‑1 (MQ=255)
                          ttATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                   <  1:1639082/68‑1 (MQ=255)
                          ttATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                   <  1:3413507/68‑1 (MQ=255)
                            aTCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                                >  1:1906949/1‑69 (MQ=255)
                                 aGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                            <  1:2653331/68‑1 (MQ=255)
                                 aGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                            <  1:2693531/68‑1 (MQ=255)
                                 aGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                            <  1:3041666/68‑1 (MQ=255)
                                     aCCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                       >  1:108918/1‑69 (MQ=255)
                                     aCCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                       >  1:2375498/1‑69 (MQ=255)
                                     aCCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                       >  1:939128/1‑69 (MQ=255)
                                     aCCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                       >  1:1357700/1‑69 (MQ=255)
                                     aCCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                       >  1:2387856/1‑69 (MQ=255)
                                     aCCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                       >  1:1389917/1‑69 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAg                                           >  1:2374241/1‑43 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGgcg                             >  1:2111118/1‑57 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:2981547/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1110411/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1145249/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1164123/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:906636/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1241400/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1265498/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:642219/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:555262/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:477578/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1504531/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1551623/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:2986738/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:2053595/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1552188/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1856300/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:2025273/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:2453275/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:1759232/1‑68 (MQ=255)
                                           cAGCATCACTTTGCCGCGCATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                  >  1:2392200/1‑68 (MQ=255)
                                                tCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaa             >  1:2703126/1‑68 (MQ=255)
                                                tCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaa             >  1:2122798/1‑68 (MQ=255)
                                                tCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaa             >  1:3238232/1‑68 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2566610/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:645479/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1382327/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1977607/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:324063/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:3211359/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2388548/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:915395/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2136068/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:1731434/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2665666/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:175446/43‑1 (MQ=255)
                                                  aCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                    <  1:2477704/43‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:2177255/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:965556/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:1978018/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:808164/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:754994/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:1086070/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:1861024/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:625958/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:615514/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:2382791/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:454914/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:1497052/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:182337/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:3361908/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:152486/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:2975074/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:2785739/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:163889/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:275928/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:1668217/69‑1 (MQ=255)
                                                          gcgcATCTCAGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTa  <  1:25964/69‑1 (MQ=255)
                                                                   |                                                           
GATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA  >  minE/1344353‑1344479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: