| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,345,321 | C→T | 36.8% | G177S (GGT→AGT) | wza ← | lipoprotein required for capsular polysaccharide translocation through the outer membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,345,321 | 0 | C | T | 36.8% | 19.9 / 20.0 | 38 | G177S (GGT→AGT) | wza | lipoprotein required for capsular polysaccharide translocation through the outer membrane |
| Reads supporting (aligned to +/- strand): ref base C (7/17); new base T (6/8); total (13/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
GCGTCGAGAATAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATG > minE/1345260‑1345385 | gCGTCGAGAATGGTCATAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:1877423/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCTGAGatt < 1:6779/69‑2 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:2395049/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:651214/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:573838/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:506962/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:44546/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:3072901/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:1977678/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:1798335/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:1353096/69‑1 (MQ=255)gCGTCGAGAATGGTCAGAGGCACGTTGGTAATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGata < 1:1354011/69‑1 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCtttt > 1:1520224/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCtttt > 1:1636109/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCtttt > 1:499713/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCtttt > 1:3361740/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCtttt > 1:3221963/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCtttt > 1:2905486/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCtttt > 1:1116152/1‑69 (MQ=255) tAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCtttt > 1:303649/1‑69 (MQ=255) gTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCtttt > 1:1074528/1‑67 (MQ=255) gTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCtttt > 1:2909805/1‑67 (MQ=255) gTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCtttt > 1:1431375/1‑67 (MQ=255) ggTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAggcggc < 1:33222/69‑1 (MQ=255) ggTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAggcggc < 1:1997990/69‑1 (MQ=255) aTCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGat > 1:2995150/1‑68 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTTACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:1414535/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGGCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:1205296/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:2345525/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:379086/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:1985007/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:1150999/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:1436200/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:74194/69‑1 (MQ=255) cGCCTGTTGACCGGATTTATTCACCTGA‑CTGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:209541/68‑1 (MQ=255) gTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAggcggc < 1:766859/58‑1 (MQ=255) tGACCGGATTTATTCACCTGACTGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATAtt < 1:3119726/62‑1 (MQ=255) tGACCGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATg > 1:2897049/1‑69 (MQ=255) | GCGTCGAGAATAGTCAGTGGCACGTTGGTGATCGCCTGTTGACCGGATTTATTCACCTGACCGGAGATATAGGCCTTTTGTGAGCGGAAGGCGGCGATATTAACGTCCACCTGCGGGTCAGCGATG > minE/1345260‑1345385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |