| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,350,590 | C→T | 24.9% | G78S (GGC→AGC) | dcd ← | 2'‑deoxycytidine 5'‑triphosphate deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,350,590 | 0 | C | T | 24.9% | 72.3 / 18.9 | 60 | G78S (GGC→AGC) | dcd | 2'‑deoxycytidine 5'‑triphosphate deaminase |
| Reads supporting (aligned to +/- strand): ref base C (24/21); new base T (9/6); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTGGG > minE/1350529‑1350653 | gTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCtcg > 1:2636430/1‑66 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:904955/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:1248244/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:63869/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:2682666/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:1590277/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:1634436/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:1693564/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga > 1:2007090/1‑69 (MQ=255) aCCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCga > 1:386529/1‑67 (MQ=255) gACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACg < 1:1107370/69‑1 (MQ=255) gACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACg < 1:2959335/69‑1 (MQ=255) gACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACg < 1:2081851/69‑1 (MQ=255) cACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCt < 1:1504929/69‑1 (MQ=255) cACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCt < 1:2983683/69‑1 (MQ=255) cACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCt < 1:2553101/69‑1 (MQ=255) aCCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCt < 1:3190229/68‑1 (MQ=255) ctctCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAg > 1:2937339/1‑69 (MQ=255) ctctCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAg > 1:2939417/1‑69 (MQ=255) ctctCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAg > 1:1429045/1‑69 (MQ=255) ctctCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAg > 1:2045691/1‑69 (MQ=255) ctctCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAg > 1:3330433/1‑69 (MQ=255) ctctCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAg > 1:3016719/1‑69 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAgcgc < 1:287891/67‑1 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAgcgc < 1:1559642/67‑1 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAgcgc < 1:2443360/67‑1 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAgcgc < 1:2185603/67‑1 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAgcgc < 1:3213732/67‑1 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAgcgc < 1:1802445/67‑1 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCgcgg > 1:2993860/1‑69 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCgcgg > 1:3366078/1‑69 (MQ=255) cTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCgcgg > 1:2887377/1‑69 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCtcatca > 1:1756099/1‑47 (MQ=37) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCa > 1:3031503/1‑68 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCa > 1:910937/1‑68 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCa > 1:981141/1‑68 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCAc > 1:184648/1‑69 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCAc > 1:562511/1‑69 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCAc > 1:1753519/1‑69 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCAc > 1:205677/1‑69 (MQ=255) aaGATAGAACGCCTCGCTTTCATCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCCCTCAc > 1:2125170/1‑69 (MQ=255) gATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACtt < 1:461291/69‑1 (MQ=255) gATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACtt < 1:2100345/69‑1 (MQ=255) gATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACtt < 1:2104298/69‑1 (MQ=255) aGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCAt > 1:2671721/1‑69 (MQ=255) aGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCAt > 1:822151/1‑69 (MQ=255) aGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCAt > 1:1785707/1‑69 (MQ=255) aGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCAt > 1:2346122/1‑69 (MQ=255) aGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCAt > 1:2593445/1‑69 (MQ=255) aCGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCt < 1:2645630/68‑1 (MQ=255) aCGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCt < 1:1060566/68‑1 (MQ=255) aCGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCt < 1:1846446/68‑1 (MQ=255) gCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCttt < 1:415606/68‑1 (MQ=255) gCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCttt < 1:2632924/68‑1 (MQ=255) cTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTggg < 1:2300326/69‑1 (MQ=255) cTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTggg < 1:3078551/69‑1 (MQ=255) cTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTggg < 1:3354905/69‑1 (MQ=255) cTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTggg < 1:2637038/69‑1 (MQ=255) cTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTggg < 1:2940588/69‑1 (MQ=255) cTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTggg < 1:1563165/69‑1 (MQ=255) | GTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCATCGCTCATCACGCGGTCAAGCGCGGCGCTCACTTCATCTTTGGG > minE/1350529‑1350653 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |