| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,352,188 | C→A | 50.4% | L180L (CTG→CTT) | thiD ← | bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,352,188 | 0 | C | A | 50.4% | 7.4 / 80.2 | 73 | L180L (CTG→CTT) | thiD | bifunctional hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (8/28); new base A (24/12); total (32/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGCGATCGCCCTTGTTCCAG > minE/1352125‑1352253 | ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:956395/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:381175/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:255071/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:1920456/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:265946/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:2890900/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:1240939/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:129969/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:1316220/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:1327681/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:2975290/67‑1 (MQ=255)ctgtgcggtAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCAAGATg < 1:1430091/67‑1 (MQ=255) aaaCCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCttt < 1:211659/69‑1 (MQ=255) aaaCCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCttt < 1:1664584/69‑1 (MQ=255) aaaCCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCttt < 1:1664481/69‑1 (MQ=255) aaaCCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCttt < 1:2998348/69‑1 (MQ=255) aaaCCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCttt < 1:1410518/69‑1 (MQ=255) aaaCCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCttt < 1:1226725/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2983372/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:3038996/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2735576/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:3299959/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:103979/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:3322702/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:3341823/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2543807/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2562622/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:1745975/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:181011/69‑1 (MQ=255) ttgttCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2038955/69‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATCGAGATGACCACCTTTCATTAg < 1:2398989/52‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:1710435/52‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:1170053/52‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2114218/52‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:239904/52‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2366410/52‑1 (MQ=255) tAAACAGCCAGTCCGGGCTTTGCTCATCATACAGATGACCACCTTTCATTAg < 1:2800107/52‑1 (MQ=255) aaaCAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2751719/51‑1 (MQ=255) aGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAg < 1:2403671/47‑1 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCAt < 1:2445991/36‑1 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:8390/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:1178367/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:2628207/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:2275221/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:1837944/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:1375438/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:3418746/1‑64 (MQ=255) cGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAAc > 1:115422/1‑64 (MQ=255) gCTCATCATCAAGATGCCCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:2822309/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAg > 1:3104730/1‑58 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:414201/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:1197495/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:3434398/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:2936435/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:542716/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:636513/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:640514/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:667301/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:1080702/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:3182203/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:3167638/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:3135979/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:3074242/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:141449/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:2740735/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:27029/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:2668880/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:2653891/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:259672/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:1874677/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGc > 1:231898/1‑59 (MQ=255) gCTCATCATCAAGATGACCACCTTTCATTAGCACTGCCCCACAGCCCATCGCCAACAGc > 1:1572627/1‑59 (MQ=255) atcCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGCGATCGCCCTTGTTCCAg < 1:2662286/69‑1 (MQ=255) | CGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCTTTCATTAGCACTGCGCCACAGCCCATCGCCAACAGCGATCGCCCTTGTTCCAG > minE/1352125‑1352253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |