| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,353,786 | A→T | 20.4% | R74R (CGT→CGA) | yohL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,353,786 | 0 | A | T | 20.4% | 160.7 / 34.6 | 88 | R74R (CGT→CGA) | yohL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/42); new base T (9/9); total (37/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
AATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCA > minE/1353729‑1353853 | aataaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTc < 1:2153139/69‑1 (MQ=255)aataaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTc < 1:1459794/69‑1 (MQ=255)aataaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTc < 1:1267246/69‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:2905372/68‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:2126368/68‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:3246008/68‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:1623222/68‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:1151030/68‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:303833/68‑1 (MQ=255) taaATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCa < 1:2458983/68‑1 (MQ=255) atatatGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTg < 1:2422722/68‑1 (MQ=255) atatatGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTg < 1:3293923/68‑1 (MQ=255) atatatGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTg < 1:757677/68‑1 (MQ=255) atatatGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTg < 1:816235/68‑1 (MQ=255) taagAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:1281511/66‑1 (MQ=255) taagAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:1420493/66‑1 (MQ=255) taagAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:1154752/66‑1 (MQ=255) taagAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:1517350/66‑1 (MQ=255) taagAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:2139399/66‑1 (MQ=255) taagAATCCAGCACCTTCAGAAAGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:1002490/66‑1 (MQ=255) aagAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:3025708/66‑1 (MQ=255) tGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGAt > 1:2935795/1‑69 (MQ=255) agAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:2935672/66‑1 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGtttt > 1:3087459/1‑43 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:1002510/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:3036380/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:107886/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:1889390/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:74426/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:690393/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACGAtgtg > 1:3246323/1‑69 (MQ=255) aTCCAGCACCTTCAGAACGACATCCAGATCTTCTTCTCGTTTTAGCTCATACCCCTGGTGAACGAtgtg > 1:2020106/1‑69 (MQ=255) tCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATgtgt < 1:2734531/69‑1 (MQ=255) tCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATgtgt < 1:2395657/69‑1 (MQ=255) tCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATgtgt < 1:23557/69‑1 (MQ=255) tCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATgtgt < 1:2340717/69‑1 (MQ=255) ccTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTcc > 1:2653601/1‑65 (MQ=255) ccTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTcc > 1:3133813/1‑65 (MQ=255) ccTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTcc > 1:1950860/1‑65 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:2236299/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:2348053/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:1564898/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:1426472/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:4248/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:2537768/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:3169186/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:306525/1‑68 (MQ=255) ttCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAg > 1:791958/1‑68 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:323323/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:3415199/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:2565118/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:2036273/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:111795/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:1137905/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:2370973/39‑1 (MQ=255) cAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATc < 1:2126252/39‑1 (MQ=255) aaCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAc < 1:1204897/68‑1 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:2748016/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:3386984/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:3393383/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:1405144/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:2098943/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:1353909/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:1233540/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:830876/1‑68 (MQ=255) aCGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGAcc > 1:2705088/1‑68 (MQ=255) aTCCAGATCTTCTTCTCGTTTTAGCTCATCCCCCTGGTGAACg < 1:612100/43‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:428568/69‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:1012768/69‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:96860/69‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:2909845/69‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:166025/69‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:265927/69‑1 (MQ=255) ccAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCAGTCAGATGACCTTTAATCa < 1:2704437/69‑1 (MQ=255) cAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCa < 1:3295834/68‑1 (MQ=255) gATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACtt > 1:3180132/1‑69 (MQ=255) gATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACtt > 1:2664506/1‑69 (MQ=255) gATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACtt > 1:307404/1‑69 (MQ=255) tcttctTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTcc > 1:1432851/1‑69 (MQ=255) cttcttCACGTTTTAGCTCAACCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTcc > 1:2764011/1‑68 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:2222965/67‑1 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:2979418/67‑1 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:26552/67‑1 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:3127750/67‑1 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:1776922/67‑1 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:1446374/67‑1 (MQ=255) tcACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCa < 1:1646998/67‑1 (MQ=255) cACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCa > 1:2879856/1‑69 (MQ=255) | AATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGCTCATCCCCCTGGTGAACGATGTGTTCCGTCAGATGACCTTTAATCACTTCCCGCATCA > minE/1353729‑1353853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |