| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,356,770 | A→G | 58.1% | S784S (AGT→AGC) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,356,770 | 0 | A | G | 58.1% | 47.1 / 87.3 | 119 | S784S (AGT→AGC) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (46/4); new base G (25/44); total (71/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.21e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TCCTTGTTGCTTATCAATTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCATTGACTTCATAACCAAACGTTAAT > minE/1356704‑1356836 | tCCTTGTTGCTTATCAATTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGAc > 1:2489067/1‑68 (MQ=255) taTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:673896/67‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:3336464/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:2135437/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:219480/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:2386439/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:2696206/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:2801936/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:3397103/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:3423745/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:1337719/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:1001352/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:1206098/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:1199046/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:92319/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:905013/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:119900/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACaa < 1:437786/68‑1 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAAc > 1:2660363/1‑69 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAAc > 1:1920962/1‑69 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAAc > 1:1150498/1‑69 (MQ=255) aaTTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAAc > 1:2977441/1‑69 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:1196243/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:2511311/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:3141242/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:2305260/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:1528708/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:1901040/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:2194755/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:1221167/1‑68 (MQ=255) gCCACATTAACCGATGGCGGTAATTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGcc > 1:1080600/1‑68 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:2693085/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:3361085/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:2956026/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:1912687/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:3314248/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:2659810/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:2638099/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc < 1:3098399/69‑1 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:804839/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc > 1:764061/1‑69 (MQ=255) cGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGAcc < 1:1840741/69‑1 (MQ=255) aCTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCa > 1:315718/1‑69 (MQ=255) aCTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCa > 1:1617332/1‑69 (MQ=255) aCTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCa > 1:3152147/1‑69 (MQ=255) aCTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCa > 1:465554/1‑69 (MQ=255) aCTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCa > 1:89243/1‑69 (MQ=255) aCTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCa > 1:1878420/1‑69 (MQ=255) cTTCATTGGTGCTAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:539308/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGAGAACACCAATATTATGACCATGGATATCa > 1:3073885/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAAca > 1:1774278/1‑43 (MQ=39) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:2945617/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:1097336/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:47849/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:2066708/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:423863/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:3351704/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:1392046/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:1535949/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:636197/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:2104761/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:740612/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:2599338/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:2527842/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:765475/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:850853/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:1974189/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:874709/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:222785/47‑1 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATc > 1:784994/1‑67 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:15306/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:623078/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1388273/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:348941/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1308377/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:662811/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:635818/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1876992/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1869890/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:2315661/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:2490050/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:2674093/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1569791/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:2902950/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1531107/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:3075841/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:3164143/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:3227897/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:2270864/1‑68 (MQ=255) cTTCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCAATATTATGACCATGGATATCa > 1:1411655/1‑68 (MQ=255) ttCATTGGTGCGAATAAATAACTGGCTTCCCTGGCCGACAACACCa < 1:2472329/46‑1 (MQ=255) cGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCAtt > 1:2785965/1‑59 (MQ=255) cGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCAtt > 1:1160694/1‑59 (MQ=255) taaataaCTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGatat > 1:3306812/1‑51 (MQ=255) taaataaCTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGatat > 1:1112243/1‑51 (MQ=255) taaataaCTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGatat > 1:2831606/1‑51 (MQ=255) taaataaCTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGatat > 1:3255215/1‑51 (MQ=255) taaataaCTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGatat > 1:408426/1‑51 (MQ=255) aaataaCTGACTTCCCTGGCCGACAACGCCAatat < 1:2099650/35‑1 (MQ=255) tGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCATTGACTTCATAACCAAACGTTAAt < 1:2463426/69‑1 (MQ=255) | TCCTTGTTGCTTATCAATTGCCACATTAACCGATGGCGGTACTTCATTGGTGCGAATAAATAACTGACTTCCCTGGCCGACAACGCCAATATTATGACCATGGATATCATTGACTTCATAACCAAACGTTAAT > minE/1356704‑1356836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |