| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 20,146 | C→G | 49.2% | P26P (CCC→CCG) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 20,146 | 0 | C | G | 49.2% | ‑1.2 / 82.2 | 67 | P26P (CCC→CCG) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (9/25); new base G (20/13); total (29/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCG > minE/20082‑20197 | actCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCTAACCCgg < 1:2603158/65‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:3311931/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:1109148/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:1986150/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:2521511/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:3186027/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:918004/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:2739172/67‑1 (MQ=255)aaTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:1601079/67‑1 (MQ=255)aaTCAACACTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:2292560/67‑1 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAAccc > 1:3047416/1‑64 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAAccc > 1:2592568/1‑64 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:2060799/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:700492/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:1584538/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:2844030/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:577636/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:968405/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:3288858/1‑65 (MQ=255) aTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg > 1:982970/1‑65 (MQ=255) tgaaCCCTGAATTTGCCGGAACCAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg < 1:2226482/62‑1 (MQ=255) tCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg < 1:355673/64‑1 (MQ=255) tCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg < 1:3376138/64‑1 (MQ=255) tCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCg < 1:982313/64‑1 (MQ=255) tttGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCTCGAACCCGGAATGCTGGCGCGTTg < 1:2304709/69‑1 (MQ=255) tttGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTg < 1:2691925/69‑1 (MQ=255) tttGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTg < 1:2791547/69‑1 (MQ=255) ttGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTgg < 1:888183/69‑1 (MQ=255) ttGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTg < 1:549314/68‑1 (MQ=255) cGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACt < 1:2266069/68‑1 (MQ=255) cGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACt < 1:1283375/68‑1 (MQ=255) cGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACt < 1:1648750/68‑1 (MQ=255) cGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACt < 1:2778855/68‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:2084455/69‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:388390/69‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:992164/69‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:1542276/69‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:1047663/69‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:1834420/69‑1 (MQ=255) aaaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:2040546/69‑1 (MQ=255) aaCAGGGTTCCCGATGCGTGGCGATCTCACCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:815649/68‑1 (MQ=255) cccGATGCGTGGCGATCTCGCCAAGCGCGAACCCgg < 1:1905725/36‑1 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGCACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTa > 1:1225877/1‑65 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACgg > 1:16794/1‑68 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACgg > 1:169608/1‑68 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACgg > 1:2953645/1‑68 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACgg > 1:1995898/1‑68 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:993796/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:625094/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:698547/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:884010/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:329527/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:1121240/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:1199779/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:1240134/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:1421508/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:2207895/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:2717864/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:2798419/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:3016026/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:3074654/1‑69 (MQ=255) aTGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGc > 1:3363649/1‑69 (MQ=255) gATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACt < 1:1035736/42‑1 (MQ=255) ctcGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCg > 1:2404270/1‑69 (MQ=255) cAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATTCGTGc < 1:2949751/63‑1 (MQ=255) cAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATTCGTGc < 1:1315643/63‑1 (MQ=255) cAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATTCGTGc < 1:3155561/63‑1 (MQ=255) aaGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATTCGTGc < 1:923708/62‑1 (MQ=255) aGCGGGAACCGGGAATGCTGGCGCGTTGGACtgatg < 1:1517713/36‑1 (MQ=37) | AATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCG > minE/20082‑20197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |