| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 108,755 | C→T | 48.0% | Y333Y (TAC→TAT) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 108,755 | 0 | C | T | 48.0% | 1.0 / 43.0 | 52 | Y333Y (TAC→TAT) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (11/16); new base T (9/16); total (20/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01 | |||||||||||
CTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAC > minE/108687‑108818 | cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:3218466/1‑69 (MQ=255)cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:2417503/1‑69 (MQ=255)cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:2359416/1‑69 (MQ=255)cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:1301476/1‑69 (MQ=255)cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:3122267/1‑69 (MQ=255)cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:1369641/1‑69 (MQ=255)cTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTAc > 1:1843019/1‑69 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:2832595/67‑1 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:1043745/67‑1 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:449882/67‑1 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:2511369/67‑1 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:3128195/67‑1 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:1348829/67‑1 (MQ=255) ccAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATAGAAAGATGGTGCGTACGtt < 1:2948808/67‑1 (MQ=255) aaCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:766574/69‑1 (MQ=255) aaCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:2926386/69‑1 (MQ=255) aCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:379544/68‑1 (MQ=255) aCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:490651/68‑1 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:2318796/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:3367725/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:397446/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:2637920/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:1122547/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:2133685/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:177847/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:1728159/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACATTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:778783/1‑68 (MQ=255) gTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTaaa < 1:1409904/69‑1 (MQ=255) ggCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGtt < 1:820222/42‑1 (MQ=255) ggCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATcc > 1:67398/1‑68 (MQ=255) ggCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATcc > 1:3261032/1‑68 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTg < 1:2823902/38‑1 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTg < 1:730432/38‑1 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgc > 1:1247739/1‑69 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgc > 1:1979177/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAACCCGCAGCACTGGt > 1:1192439/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:641985/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:2977258/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:3167529/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:3367919/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:1950930/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:1729074/1‑69 (MQ=255) aTCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg < 1:2909664/68‑1 (MQ=255) aTCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg < 1:1725705/68‑1 (MQ=255) aTCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg < 1:878805/68‑1 (MQ=255) gAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAga < 1:2420895/69‑1 (MQ=255) gAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAga < 1:207365/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:3316254/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2678852/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:645039/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2324581/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:1080736/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:129927/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:1888847/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2172456/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2453248/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2546544/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2722765/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2913315/69‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCtcag < 1:2228583/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:3208489/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:2805167/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:201780/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:2812195/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:1110167/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:846090/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAgactg > 1:2877730/1‑57 (MQ=255) | CTGATCCAGCTGATGAACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAC > minE/108687‑108818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |