| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,357,310 | A→G | 28.1% | N604N (AAT→AAC) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,357,310 | 0 | A | G | 28.1% | 70.8 / 29.1 | 71 | N604N (AAT→AAC) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (17/34); new base G (11/9); total (28/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GGTCAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGATCAT > minE/1357247‑1357377 | ggTCAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGa < 1:1495713/68‑1 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACt > 1:127662/1‑68 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg < 1:1859435/69‑1 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg < 1:1680940/69‑1 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg < 1:3089455/69‑1 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg > 1:3114177/1‑69 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg < 1:3122348/69‑1 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg < 1:1364666/69‑1 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg > 1:123746/1‑69 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg > 1:3408344/1‑69 (MQ=255) cAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTg > 1:1055081/1‑69 (MQ=255) aTTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGAt < 1:2741661/68‑1 (MQ=255) aTTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGAt < 1:1967859/68‑1 (MQ=255) aTTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGAt < 1:1994213/68‑1 (MQ=255) aTTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGAt < 1:500237/68‑1 (MQ=255) aTTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGAt < 1:616457/68‑1 (MQ=255) ttCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGAt < 1:1310331/67‑1 (MQ=255) tCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:221375/69‑1 (MQ=255) tCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:3291440/69‑1 (MQ=255) tCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:1555283/69‑1 (MQ=255) cccAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACt < 1:3068311/69‑1 (MQ=255) gCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCt < 1:1888516/69‑1 (MQ=255) gCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCt < 1:1591116/69‑1 (MQ=255) cTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTa < 1:1739092/69‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:288081/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:2359727/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:2590596/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:1815913/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:1693352/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:3132825/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:3219998/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:3241475/68‑1 (MQ=255) ttCATTTCCCTGATACTGATAACTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTc < 1:1253966/68‑1 (MQ=255) ttCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGAt < 1:2454205/68‑1 (MQ=255) cTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGAt < 1:2308505/64‑1 (MQ=255) ttGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:2936865/38‑1 (MQ=255) ttGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:2812197/38‑1 (MQ=255) ttGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:859506/38‑1 (MQ=255) ttGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:102531/38‑1 (MQ=255) ttGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:2009340/38‑1 (MQ=255) ttGATGACTCAGGTTGACACCATAATTGAACTGATccc < 1:1298689/38‑1 (MQ=255) gatgaCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGtatta > 1:902569/1‑68 (MQ=255) gatgaCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGtatta > 1:1990382/1‑68 (MQ=255) gatgaCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGtatta > 1:512350/1‑68 (MQ=255) gatgaCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGtat < 1:1169715/66‑1 (MQ=255) gatgaCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTACTGATAATCCCGtattat > 1:323929/1‑69 (MQ=255) gatgaCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTACTGATAATCCCGtattat > 1:319813/1‑69 (MQ=255) aCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGa > 1:393037/1‑69 (MQ=255) aCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGa > 1:1579032/1‑69 (MQ=255) aCTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGa > 1:1478538/1‑69 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCtt > 1:292841/1‑35 (MQ=16) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:398085/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:492450/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:2634376/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:3185353/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:1409225/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:1059712/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:1550407/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:302800/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCGGCTTCCAACGGTTCCTGa > 1:2170915/1‑50 (MQ=255) cTCAGGTTGACCCCATAGTTAAACTGATCCCAGCTTCCAACGGTTCCTGa > 1:1003170/1‑50 (MQ=255) gTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCa < 1:701398/67‑1 (MQ=255) gTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCa < 1:1352351/67‑1 (MQ=255) gTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCa < 1:471382/67‑1 (MQ=255) gTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCa < 1:367024/67‑1 (MQ=255) gTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCa < 1:2961393/67‑1 (MQ=255) gACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAAccc < 1:1328473/69‑1 (MQ=255) aTAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGatc > 1:537410/1‑69 (MQ=255) aTAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGatc > 1:31258/1‑69 (MQ=255) aTAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGatc > 1:2083462/1‑69 (MQ=255) aTAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGatc > 1:2201617/1‑69 (MQ=255) aTTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGatcat > 1:1074891/1‑68 (MQ=255) | GGTCAAATTCGCCCCAGCTGTCGTTTCATTTCCCTGATGTTGATGACTCAGGTTGACACCATAATTGAACTGATCCCGACTCCCTACTGTTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGATCAT > minE/1357247‑1357377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |