| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 108,761 | T→C | 42.6% | R335R (CGT→CGC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 108,761 | 0 | T | C | 42.6% | 14.1 / 45.2 | 61 | R335R (CGT→CGC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base T (12/23); new base C (9/17); total (21/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGA > minE/108702‑108825 | aaCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:766574/69‑1 (MQ=255)aaCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:2926386/69‑1 (MQ=255) aCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:379544/68‑1 (MQ=255) aCGAAACCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACActtc < 1:490651/68‑1 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:2637920/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:3367725/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:2318796/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:397446/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:2133685/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:177847/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:1728159/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACCTTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:1122547/1‑68 (MQ=255) aaCCGTTGACGGCGATTACCAGACATTCAAATCGAAAGATGGTGCGTATGTTCGCGAACACTTCTTCg > 1:778783/1‑68 (MQ=255) gTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTaaa < 1:1409904/69‑1 (MQ=255) ggCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATcc > 1:67398/1‑68 (MQ=255) ggCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATcc > 1:3261032/1‑68 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTg < 1:2823902/38‑1 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTg < 1:730432/38‑1 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgc > 1:1979177/1‑69 (MQ=255) ccAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgc > 1:1247739/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAACCCGCAGCACTGGt > 1:1192439/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:1729074/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:3367919/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:3167529/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:2977258/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:641985/1‑69 (MQ=255) cAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGt > 1:1950930/1‑69 (MQ=255) aTCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg < 1:2909664/68‑1 (MQ=255) aTCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg < 1:878805/68‑1 (MQ=255) aTCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTg < 1:1725705/68‑1 (MQ=255) gAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAga < 1:2420895/69‑1 (MQ=255) gAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAga < 1:207365/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:3316254/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:645039/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2913315/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2722765/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:129927/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:1080736/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:1888847/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2678852/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2172456/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2546544/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2453248/69‑1 (MQ=255) gCGTATGTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2324581/69‑1 (MQ=255) tgTTCGCGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGAc < 1:2746511/63‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCtcag < 1:2228583/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:1110167/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:846090/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:201780/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:3208489/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:2805167/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCgcag < 1:2812195/41‑1 (MQ=255) cGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAgactg > 1:2877730/1‑57 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:3332940/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:2568045/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:1943984/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:1480567/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:1134691/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:848241/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:851164/69‑1 (MQ=255) ttCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGa < 1:852172/69‑1 (MQ=255) | AACGAAACCGTTGACGGCGACTACCAGACCTTCAAATCGAAAGATGGTGCGTACGTTCGTGAACACTTCTTCGGTAAATATCCTGAAACCGCAGCACTGGTTGCAGACTGGACTGACGAGCAGA > minE/108702‑108825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |