| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,357,714 | C→T | 34.0% | V470I (GTC→ATC) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,357,714 | 0 | C | T | 34.0% | 104.4 / 71.5 | 129 | V470I (GTC→ATC) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (54/31); new base T (37/7); total (91/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 | |||||||||||
Only 100 of 129 total aligned reads displayed.
TTGGCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTA > minE/1357653‑1357780 | ttGGCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtt > 1:2670122/1‑68 (MQ=255)ttGGCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtt > 1:175884/1‑68 (MQ=255)ttGGCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtt > 1:1630075/1‑68 (MQ=255) ggCGGAAAAGCTATTTTTGCGTCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtttt < 1:140828/68‑1 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtttt < 1:1549830/68‑1 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtttt < 1:957230/68‑1 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtttt > 1:1051144/1‑68 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtttt < 1:2555571/68‑1 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGtttt < 1:2726034/68‑1 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTtc > 1:1503321/1‑69 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTtc > 1:1581392/1‑69 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTtc > 1:2099141/1‑69 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTtc > 1:3368981/1‑69 (MQ=255) ggCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTtc > 1:831744/1‑69 (MQ=255) aaaGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACg > 1:114496/1‑69 (MQ=255) aaaGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACg > 1:2304447/1‑69 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:49512/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:2924930/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:2909996/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:2733615/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:2440774/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:2554164/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:814875/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:1053926/69‑1 (MQ=255) aaaTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTg < 1:832059/69‑1 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGc > 1:3008073/1‑50 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:53392/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:2475916/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:1078257/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:1208295/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:3416309/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:2758780/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:2682210/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:829353/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:101689/1‑66 (MQ=255) aTCGTTCTGGTAATAATCGGCAATGTCATAAACATCGTTTTCATCACGGCGATAATTATCTTTAtt > 1:1107698/1‑66 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:1993475/69‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:3149362/69‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:1494450/69‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:3333752/69‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:888173/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:2260476/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:2514337/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:257050/68‑1 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAAt > 1:869868/1‑41 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACg > 1:3246537/1‑64 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:355597/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3199891/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3324299/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3435005/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2521419/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:471511/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:691348/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:761320/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:965058/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2538497/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1009725/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1116903/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1211199/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1225090/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1294988/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:131130/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1441863/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:144747/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1939048/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2036950/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2217821/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2272103/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2294010/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2284952/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2482539/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1005576/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:2407509/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:1661378/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:1619331/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:1569833/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:2064948/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:3258781/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:2763998/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:3339207/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:77995/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCAAAACGTg > 1:3241623/1‑56 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:3175851/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:1664257/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:2808984/61‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGt > 1:3039693/1‑68 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGt > 1:3193636/1‑68 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1630985/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:761241/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1405258/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:1704472/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1870769/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:238934/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:2910083/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1116968/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:870463/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:2943730/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:926338/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1018319/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:258333/1‑69 (MQ=255) | TTGGCGGAAAAGCTATTTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTA > minE/1357653‑1357780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |