| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,357,757 | A→T | 45.7% | V455V (GTT→GTA) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,357,757 | 0 | A | T | 45.7% | 21.1 / 106.0 | 94 | V455V (GTT→GTA) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (30/21); new base T (36/7); total (66/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGA > minE/1357693‑1357825 | aataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCaaa < 1:3299561/66‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:1993475/69‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:1494450/69‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:3149362/69‑1 (MQ=255) ataatCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTg < 1:3333752/69‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:2546507/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:257050/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:1589436/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:2260476/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:2514337/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:888173/68‑1 (MQ=255) aatCGGCAATGTCATAGATATCGTTTTCATAACGGCGATAATTATCTTTATTGTTTGCCCATACGTGa < 1:229277/68‑1 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACg > 1:3246537/1‑64 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2482539/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2620685/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2521419/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1005576/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2036950/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2294010/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2217821/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2284952/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2272103/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2538497/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2499493/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2909104/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:2925923/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3199891/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3235754/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3324299/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:3435005/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:355597/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:471511/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:691348/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:761320/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:860386/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:965058/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1939048/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1441863/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1418098/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:131130/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:144747/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1294988/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1499353/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1225090/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1211199/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1116903/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:104223/1‑69 (MQ=255) atCGGCAATGTCATAGATATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCATACGTGATc > 1:1009725/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:2407509/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:1661378/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:1619331/1‑69 (MQ=255) atCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATc > 1:1569833/1‑69 (MQ=255) gTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGt > 1:792135/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:3339207/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:1174116/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:355770/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:3258781/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:77995/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:2064948/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTc > 1:2763998/1‑69 (MQ=255) tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCAAAACGTg > 1:3241623/1‑56 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGTTCGTTa < 1:3381877/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:3175851/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:2808984/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:1664257/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:1632649/61‑1 (MQ=255) aTAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTa < 1:751005/61‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGt > 1:3039693/1‑68 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGt > 1:3193636/1‑68 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:2177174/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:926338/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1116968/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:1704472/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:761241/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1833056/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1870769/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:2910083/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:870463/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1018319/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa < 1:2943730/69‑1 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:238934/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1405258/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1630985/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:258333/1‑69 (MQ=255) gACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTa > 1:1262618/1‑69 (MQ=255) aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAAt > 1:368067/1‑68 (MQ=255) gTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCAc > 1:3309974/1‑68 (MQ=255) atcaCGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGcgacg < 1:2911681/68‑1 (MQ=255) atcaCGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGcgacg < 1:1237190/68‑1 (MQ=255) aaTTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAgg > 1:113075/1‑69 (MQ=255) aaTTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAgg > 1:1588354/1‑69 (MQ=255) aTCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgccgc < 1:1749414/69‑1 (MQ=255) aTCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgccgc < 1:497451/69‑1 (MQ=255) aTCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgccgc < 1:1675106/69‑1 (MQ=255) aTCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgccgc < 1:935240/69‑1 (MQ=255) aaCGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCg > 1:1071688/1‑68 (MQ=255) aaCGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGa > 1:3379587/1‑69 (MQ=255) | AATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGA > minE/1357693‑1357825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |