| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,357,820 | C→T | 30.2% | T434T (ACG→ACA) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,357,820 | 0 | C | T | 30.2% | 89.8 / 41.1 | 93 | T434T (ACG→ACA) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (28/36); new base T (5/23); total (34/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATC > minE/1357757‑1357882 | aaCGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCg > 1:1071688/1‑68 (MQ=255)aaCGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGa > 1:3379587/1‑69 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCTACGtt < 1:439054/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2885034/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1616919/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:3026506/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1673263/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:3020235/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2906455/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1761163/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1054792/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1924974/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2858691/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2475275/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2180084/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2375158/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:2353713/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:605533/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:3403184/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1415762/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1300621/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1491436/69‑1 (MQ=255) tGATCATTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCCCCTGACCAAAACGTGTCGACGtt < 1:1163253/69‑1 (MQ=255) aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTg > 1:2715500/1‑69 (MQ=255) cattAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:1469234/63‑1 (MQ=255) cGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTgg < 1:2178858/68‑1 (MQ=255) cGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTgg < 1:1993335/68‑1 (MQ=255) tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTc > 1:1430237/1‑68 (MQ=255) tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTc > 1:1816303/1‑68 (MQ=255) cGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttg < 1:2068739/69‑1 (MQ=255) gTAATCACGCGACGAATATCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttgtt < 1:2419176/69‑1 (MQ=255) gTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttgt < 1:46135/68‑1 (MQ=255) gTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttgt < 1:1863712/68‑1 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGTTTGGCTCACAAATTtgttgt > 1:293433/1‑69 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGTTTGGCTCACAAATTtgttgt > 1:1421275/1‑69 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGTTTGGCTCACAAATTtgttgt > 1:30618/1‑69 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGTTTGGCTCACAAATTtgttgt > 1:2193697/1‑69 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGTTTGGCTCACAAATTtgttgt > 1:1579173/1‑69 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTtgttgt > 1:485846/1‑69 (MQ=255) aaTCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTtgttgt > 1:240731/1‑69 (MQ=255) gcgACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCaa < 1:1346464/69‑1 (MQ=255) acgaATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:160846/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:422624/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:2652034/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:493852/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:313292/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:2330312/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:1002443/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:2098635/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:1367025/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:2037176/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:1631766/69‑1 (MQ=255) acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:1986581/69‑1 (MQ=255) cgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTg < 1:933242/69‑1 (MQ=255) cgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTg < 1:122589/69‑1 (MQ=255) cgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTg < 1:1539028/69‑1 (MQ=255) cgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTg < 1:3075172/69‑1 (MQ=255) cgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTg < 1:1694290/69‑1 (MQ=255) gaATAACGCCAGGCCGCCAGACCAAAACGTGTCGACGtt < 1:512232/39‑1 (MQ=255) aaTAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGAt < 1:3254465/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:2397634/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:1466985/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:3221242/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:2703848/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:2987712/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:2221905/69‑1 (MQ=255) aaCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAAc < 1:1746585/69‑1 (MQ=255) gACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAaca < 1:489881/69‑1 (MQ=255) gACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAaca < 1:173544/69‑1 (MQ=255) gACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAaca < 1:3231808/69‑1 (MQ=255) ccAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAttt < 1:1079544/46‑1 (MQ=255) ccAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAAcaca > 1:1345872/1‑69 (MQ=255) ccAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAAcaca > 1:198826/1‑69 (MQ=255) cAAAACGCGTCGACGTTTGGCTGACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt > 1:3123083/1‑69 (MQ=255) cAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt > 1:1872647/1‑69 (MQ=255) cAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt > 1:1247409/1‑69 (MQ=255) cAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt > 1:1358169/1‑69 (MQ=255) cAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt > 1:1661957/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:3391514/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:2453151/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:3421395/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:2060020/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:3376248/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:461159/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:318138/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:2599940/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:3099408/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:2001878/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:1649325/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:53283/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:872676/1‑69 (MQ=255) aaaaCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:2967595/1‑69 (MQ=255) aaaaCGAGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATc > 1:436641/1‑69 (MQ=255) | AACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATC > minE/1357757‑1357882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |