| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,359,713 | A→G | 34.2% | N48N (AAT→AAC) ‡ | yehC ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,359,713 | 0 | A | G | 34.2% | 77.0 / 62.3 | 108 | N48N (AAT→AAC) ‡ | yehC | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (46/25); new base G (19/18); total (65/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
ATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAC > minE/1359647‑1359775 | atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTa > 1:1569024/1‑67 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAtt > 1:180147/1‑69 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:1026683/1‑68 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:2722972/1‑68 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:2766114/1‑68 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:1891203/1‑68 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:612607/1‑68 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:66356/1‑68 (MQ=255)atcaATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAt > 1:1515489/1‑68 (MQ=255) aaTCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAtttt > 1:2879289/1‑68 (MQ=255) aaTCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAtttt > 1:204522/1‑68 (MQ=255) aaTCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTc > 1:1169537/1‑69 (MQ=255) aaTCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTc > 1:244046/1‑69 (MQ=255) aaTCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTc > 1:90685/1‑69 (MQ=255) tCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCt < 1:1887216/68‑1 (MQ=255) tCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCt < 1:2601634/68‑1 (MQ=255) tCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCt < 1:1362492/68‑1 (MQ=255) cACGCCTGCAGCAGCTAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAttt > 1:1932592/1‑63 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTAttt > 1:1484451/1‑63 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:2094495/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:2530418/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:985391/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:2275211/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:2905006/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:2069513/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGcc > 1:1560037/1‑69 (MQ=255) cACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTATAtt > 1:862990/1‑62 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:2122658/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:127003/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:131029/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:2655429/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:548162/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:135001/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:2898996/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:911756/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:3092483/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:578916/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:3288472/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:332925/69‑1 (MQ=255) aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:1924127/69‑1 (MQ=255) agaaCGGTTTGCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:1392413/68‑1 (MQ=255) agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAATTAATAcgcg < 1:1989209/68‑1 (MQ=255) agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:775827/68‑1 (MQ=255) agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:889278/68‑1 (MQ=255) agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:536266/68‑1 (MQ=255) gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:287325/69‑1 (MQ=255) gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:1099209/69‑1 (MQ=255) gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:2517420/69‑1 (MQ=255) gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:1431142/69‑1 (MQ=255) tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:195591/68‑1 (MQ=255) tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:887343/68‑1 (MQ=255) tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:1616914/68‑1 (MQ=255) tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:595272/68‑1 (MQ=255) tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:2970110/68‑1 (MQ=255) tcatcaACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc > 1:741699/1‑64 (MQ=255) tcatcaACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc > 1:452147/1‑64 (MQ=255) caACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCgg < 1:1375628/68‑1 (MQ=255) caACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCgg < 1:2416402/68‑1 (MQ=255) aaCTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa > 1:3127385/1‑69 (MQ=255) aaCTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa > 1:549915/1‑69 (MQ=255) aaCTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa > 1:2902060/1‑69 (MQ=255) aaCTGCACAATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGc > 1:2518633/1‑68 (MQ=255) aaCTGCA‑CATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCTTa < 1:3108028/52‑1 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:2772161/1‑67 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:3250080/1‑67 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:3345353/1‑67 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:678259/1‑67 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:1451263/1‑67 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:2419513/1‑67 (MQ=255) gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:2841305/1‑67 (MQ=255) cACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaag < 1:3080128/69‑1 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACaa > 1:2665076/1‑49 (MQ=37) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2778086/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:436999/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:191225/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:1822103/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:1785451/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2709908/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:1948694/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2187782/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2383504/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2865533/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2366736/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2803284/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2237236/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2242891/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:35341/1‑53 (MQ=255) aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2272617/1‑53 (MQ=255) aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:91890/41‑1 (MQ=255) aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:1342583/1‑69 (MQ=255) aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:285979/1‑69 (MQ=255) aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:1235336/1‑69 (MQ=255) aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:2924633/1‑69 (MQ=255) ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:711363/69‑1 (MQ=255) ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:1107913/69‑1 (MQ=255) ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:75621/69‑1 (MQ=255) ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:1675215/69‑1 (MQ=255) ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:3220594/69‑1 (MQ=255) ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:3354443/69‑1 (MQ=255) cttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc > 1:659065/1‑67 (MQ=255) | ATCAATCCACGCCTGCAGCAGCGAAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAC > minE/1359647‑1359775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |