| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 108,941 | C→T | 42.8% | Y395Y (TAC→TAT) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 108,941 | 0 | C | T | 42.8% | 10.8 / 27.9 | 42 | Y395Y (TAC→TAT) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (19/5); new base T (13/5); total (32/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGT > minE/108878‑109007 | cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:1155347/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:3411239/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:3374810/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:3176143/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:2924120/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:282914/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:2667870/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:26087/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:2469679/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:1916418/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:1770419/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATACCATTAAAGGTTATGGCAt > 1:1638299/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACGGTAATCCTTGCTCATAACATTAAAGGTTATGGCa > 1:1989052/1‑68 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAt > 1:2020340/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAt > 1:3382085/1‑69 (MQ=255)cAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAt > 1:2873249/1‑69 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:3323826/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:317928/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:1171331/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:2888403/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:1420819/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:2505768/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:1564038/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:870585/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:2189789/1‑67 (MQ=255) aaaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGc > 1:1584721/1‑67 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTa < 1:2655107/69‑1 (MQ=255) tGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGtt > 1:199327/1‑67 (MQ=255) tGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTa > 1:2833301/1‑68 (MQ=255) tCATACCATTAAAGGTTATGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg < 1:1104752/67‑1 (MQ=255) tCATACCATTAAAGGTTATGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg < 1:1364345/67‑1 (MQ=255) tCATACCATTAAAGGTTATGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg < 1:3372873/67‑1 (MQ=255) cATACCATTAAAGGTTATGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg < 1:1770563/66‑1 (MQ=255) cATACCATTAAAGGTTATGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAg < 1:3040354/66‑1 (MQ=255) ccATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATg > 1:3246018/1‑68 (MQ=255) ccATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATg > 1:802053/1‑68 (MQ=255) ccATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATg > 1:887236/1‑68 (MQ=255) gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGaa > 1:1187126/1‑61 (MQ=255) tACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGgt < 1:1311134/69‑1 (MQ=255) tACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGgt < 1:1422207/69‑1 (MQ=255) tACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGgt < 1:505323/69‑1 (MQ=255) tACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGgt < 1:2384167/69‑1 (MQ=255) | CAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGT > minE/108878‑109007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |