Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,359,918 A→G 43.7% S172S (TCT→TCC yehD ← predicted fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,359,9180AG43.7% 11.5 / 49.6 64S172S (TCT→TCCyehDpredicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (7/29);  new base G (10/18);  total (17/47)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTAAA  >  minE/1359856‑1359980
                                                              |                                                              
tGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGAt                                                          <  1:1172455/69‑1 (MQ=255)
tGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGAt                                                          <  1:2359339/69‑1 (MQ=255)
tGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGAt                                                          <  1:157809/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGAt                                                          >  1:786952/1‑67 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:2583832/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:1833193/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:2196346/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:1704120/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:2209535/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:1541695/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        >  1:2375997/1‑69 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:2617372/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:2665857/69‑1 (MQ=255)
  tttATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:380687/69‑1 (MQ=255)
   ttattaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATaa                                                        <  1:2766870/68‑1 (MQ=255)
    tattaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAaca                                                      <  1:1039469/69‑1 (MQ=255)
    tattaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAaca                                                      <  1:82902/69‑1 (MQ=255)
    tattaCCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAac                                                       <  1:998434/68‑1 (MQ=255)
               gaAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGAt                                           <  1:2696815/69‑1 (MQ=255)
               gaAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACAAATCACCTGAt                                           <  1:3376418/69‑1 (MQ=255)
                 aaCTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTg                                         <  1:2436670/69‑1 (MQ=255)
                 aaCTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTg                                         <  1:1594939/69‑1 (MQ=255)
                  aCTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGc                                        <  1:689330/69‑1 (MQ=255)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:2852453/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:2711847/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:2717500/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:283959/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:147317/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:25040/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:1603720/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:936491/1‑40 (MQ=38)
                              atttattAATCCTGCGCCACACGCATAATCAGGGAAGATa                                                         >  1:1531572/1‑40 (MQ=38)
                              atttattAATCCTGCGACACACGCATAATCAGGGAAGATa                                                         >  1:340637/1‑40 (MQ=37)
                                 tattaATCCTGCGCCACACGCATAACCAGAGAAGAt                                                          >  1:367791/1‑36 (MQ=255)
                                 tattaATCCTGCGCCACACGCATAACCAGAGAAGAt                                                          >  1:2709083/1‑36 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:3209105/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:578528/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:3419352/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:3338172/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:1407325/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:1467519/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:773754/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:2458242/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:1102490/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:2227427/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:1747141/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:2195027/69‑1 (MQ=255)
                                               cacaCGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:2057622/69‑1 (MQ=255)
                                               cacaCGAATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGTTAttt           <  1:3054152/69‑1 (MQ=255)
                                                actcGCATAATGAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:1229359/65‑1 (MQ=255)
                                                acacGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:648645/68‑1 (MQ=255)
                                                acacGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:986891/68‑1 (MQ=255)
                                                acacGCATAATCAGGGAAGATAACACATCACCTGAATTCACTGAGTTATCTGTCAGTTTCAGGTAttt           <  1:1380428/68‑1 (MQ=255)
                                                      aTAACCAGAGAATATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:3185621/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:1893926/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:2589442/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:2439852/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:841661/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:845640/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:2311701/69‑1 (MQ=255)
                                                      aTAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTa    <  1:2006939/69‑1 (MQ=255)
                                                       tAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCa                  >  1:2408352/1‑54 (MQ=255)
                                                         aCCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTaaa  >  1:804097/1‑68 (MQ=255)
                                                         aCCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTaaa  >  1:1012972/1‑68 (MQ=255)
                                                              |                                                              
TGTTTATTACCTGGAGAAACTTACAATTTTATTTATTAATCCTGCGCCACACGCATAACCAGAGAAGATAACACATCACCTGATTGCACTGAGTTATCTGTCAGTTTCAGGTATTTTGCTTTAAA  >  minE/1359856‑1359980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: