| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,361,205 | T→A | 50.5% | intergenic (‑198/+65) | yehE ← / ← mrp | hypothetical protein/antiporter inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,361,205 | 0 | T | A | 50.5% | 5.3 / 146.6 | 121 | intergenic (‑198/+65) | yehE/mrp | hypothetical protein/antiporter inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (11/49); new base A (10/51); total (21/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAG > minE/1361138‑1361273 | gagTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACt < 1:1754273/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGTCAAAAACTaa < 1:3299188/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:2479916/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:1828694/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:1538186/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:2572574/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:2195915/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:1868170/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:818827/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:1367196/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:3245553/68‑1 (MQ=255) gTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa < 1:1462208/68‑1 (MQ=255) tCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGc < 1:95464/62‑1 (MQ=255) tCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGc < 1:3160421/62‑1 (MQ=255) tCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGc < 1:1240059/62‑1 (MQ=255) tCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGc < 1:870831/62‑1 (MQ=255) ccGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGt > 1:876673/1‑69 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1954960/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:2904039/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:281574/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1837117/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:3255228/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1621093/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1084957/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1258470/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:84475/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:718615/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:376908/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:447699/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1559101/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:57965/69‑1 (MQ=255) atatTCGCAGCATTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACAtct < 1:1484219/69‑1 (MQ=255) tatTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATc > 1:1649863/1‑56 (MQ=255) tatTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACAtctc > 1:1852856/1‑69 (MQ=255) tatTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACAtctc > 1:3389299/1‑69 (MQ=255) ttCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTaa > 1:714473/1‑43 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:449861/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:2040051/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:168495/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:2722616/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:3272652/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:1256015/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCgg > 1:2425795/1‑69 (MQ=255) gcagcaTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGCg > 1:107406/1‑68 (MQ=255) aTTAAATAAGTGTTCATTAATGCGGCAAAAACAAATACACCGCATCTATGTAACAtct < 1:931049/58‑1 (MQ=255) ttAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTa > 1:1597462/1‑67 (MQ=255) tAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCgg > 1:375344/1‑58 (MQ=255) tAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATgaaga > 1:1145508/1‑68 (MQ=255) tgtTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAAc < 1:2261479/69‑1 (MQ=255) tgtTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAAc < 1:3077033/69‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:2159584/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:2028140/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:54885/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:296737/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:2050905/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:594506/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:2703541/68‑1 (MQ=255) cATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAg < 1:935912/68‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:1870833/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:3327026/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:1269574/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:1201648/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:2976793/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:2234728/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATgg < 1:2366854/69‑1 (MQ=255) aaTGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATg > 1:1034249/1‑68 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:1580738/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:3436437/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:359548/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2422338/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:1532765/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:485324/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:1444585/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:683467/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:1378690/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:849111/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2597768/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:3351284/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2650341/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2656601/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:270829/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2732323/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2054002/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:3222991/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2981412/44‑1 (MQ=255) aaTGCGGCAAAAACAAATACACCGCATCAATGTAACATCTCTGc < 1:2899092/44‑1 (MQ=255) aaTGCGGCAAAAAAAAATACACCGCATCAATGTAACATCTCTGc < 1:2341433/44‑1 (MQ=38) tGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGt < 1:3308082/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:2865017/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:1440337/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:804715/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:1294681/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:1626134/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:2648596/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:1612804/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:2526768/68‑1 (MQ=255) gCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:3391193/68‑1 (MQ=255) cGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGtt < 1:1731890/67‑1 (MQ=255) tAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAg < 1:467874/69‑1 (MQ=255) tAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAg < 1:1676166/69‑1 (MQ=255) | GAGTTTCGGACGAAGGTCCGCAGAATATTCGCAGTATTAAATAAGTGTTCATTAATGCGGCAAAAACTAATACACCGCATCAATGTAACATCTCTGCGGTGTATGAAGAATAACCAGAATGGTTAATAGGCGTTAG > minE/1361138‑1361273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |