| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,363,569 | A→C | 25.3% | A353A (GCA→GCC) | metG → | methionyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,363,569 | 0 | A | C | 25.3% | 94.7 / 33.6 | 71 | A353A (GCA→GCC) | metG | methionyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (24/29); new base C (11/7); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGG > minE/1363508‑1363636 | aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:1959627/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:2582510/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:1300954/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:1347536/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:2505751/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:236707/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:3405702/3‑69 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGc > 1:2371615/3‑68 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGc > 1:1790585/3‑68 (MQ=255)aaaTGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGc > 1:1696437/3‑68 (MQ=255)aaaTGTCCAAGTCTCGCAGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCCGACAGcc > 1:2040622/3‑69 (MQ=255)aGATGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGcc < 1:440392/69‑1 (MQ=255)aGATGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGcc < 1:816966/69‑1 (MQ=255)aGATGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGcc < 1:2803512/69‑1 (MQ=255) tCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGt < 1:1751602/69‑1 (MQ=255) tCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGt < 1:492309/69‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGAGAGCCTGCGTtactactac < 1:1788614/45‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac > 1:2497810/1‑45 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:387958/45‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac > 1:2989953/1‑45 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac > 1:3091139/1‑45 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac > 1:3018303/1‑45 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:1788455/45‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:733294/45‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:797293/45‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:137118/45‑1 (MQ=255) aCCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:2376738/45‑1 (MQ=255) aCCTCGCTGAATCATTTTGACGCAGACAGCCTGCGTtactactac < 1:2156542/45‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGATACTCTCTTCGCGCATtgat < 1:2067024/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCAt > 1:2180013/1‑65 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2949547/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:278289/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2920472/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:985034/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:3095406/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:427771/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:443707/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:480946/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:868116/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:932088/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:977979/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:1054434/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:1660486/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:2043516/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:208346/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:1872226/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2168578/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:1832235/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:1831774/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2183868/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:1549429/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat < 1:1519261/69‑1 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2568113/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2655664/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATtgat > 1:2746314/1‑69 (MQ=255) tGGCTGAATCATTTTGACGCAGACAGCCTGCGTAACTACTACACTGCGaaa > 1:1126227/1‑51 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:2744765/69‑1 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:727663/69‑1 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:926494/69‑1 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:2272733/69‑1 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:2436936/69‑1 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:2535844/69‑1 (MQ=255) ggCTGAATCATTTTGACGCCGACAGCCTGCGCTACTACTACACCGCCAAACTGTCTTCGCGCATtgatg < 1:2806270/69‑1 (MQ=255) aTCATTTTGACGCAGAAAGCCTGCGTtactactac < 1:3214143/35‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:2119294/69‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:3210351/69‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:3143572/69‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:2313909/69‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:1447670/69‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:1256509/69‑1 (MQ=255) cAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTgg < 1:1227216/69‑1 (MQ=255) | AGATGTCCAAGTCTCGCGGCACCTTTATTAAAGCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGG > minE/1363508‑1363636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |