| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 109,697 | C→T | 57.7% | H647H (CAC→CAT) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 109,697 | 0 | C | T | 57.7% | 21.0 / 59.1 | 78 | H647H (CAC→CAT) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (16/17); new base T (21/24); total (37/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCAT > minE/109630‑109765 | tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCcaca > 1:2141555/1‑69 (MQ=255)tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCcaca > 1:440283/1‑69 (MQ=255) ggcGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGGCACAGCCATATTCAGTCGCTGACTa < 1:2333988/67‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1782281/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:2683361/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:2632199/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:2623350/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:2591336/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:272160/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:2754872/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:3042182/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1927027/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:191834/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1736459/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1548228/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1495719/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1465699/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1155897/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:800740/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1159366/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:745936/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:660666/69‑1 (MQ=255) gTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGACATATTCAGTCGCTGACTa < 1:3204994/69‑1 (MQ=255) tCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:2104530/68‑1 (MQ=255) tCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:1893507/68‑1 (MQ=255) cTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGt > 1:404299/1‑69 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTa > 1:281871/1‑68 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTAt > 1:594458/1‑69 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTAt > 1:1723985/1‑69 (MQ=255) gAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACt < 1:1903161/48‑1 (MQ=255) aaGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACt < 1:2082999/47‑1 (MQ=255) cTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:1247469/41‑1 (MQ=255) cTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:827065/41‑1 (MQ=255) cTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:821407/41‑1 (MQ=255) cTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:3035090/41‑1 (MQ=255) cTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:2285185/41‑1 (MQ=255) cTGCAGCACGAAGATGGTCACAGCCACATTCAGGCGCTGAc < 1:3430695/41‑1 (MQ=255) tGCAGCACGAAGATGTTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:3167310/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:995699/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:374611/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:2805782/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:3423400/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:496544/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:3322214/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:2551500/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:3226532/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:3318216/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:560533/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:102906/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:1387919/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:1614922/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:1987368/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:1230070/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg > 1:1156079/1‑69 (MQ=255) tGCAGCACGAAGATGGTCACAGCCATATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCg > 1:1381188/1‑68 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:2532788/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:2928049/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:578417/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:2119209/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:1152543/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:3249697/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:1727382/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:1235490/67‑1 (MQ=255) gcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct < 1:3376792/67‑1 (MQ=255) cGAAGATGGTCACAGCCATATTCAGTCGCTGACTa < 1:19741/35‑1 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGCCCCGGCTTACGCTTACGAAg > 1:2776212/1‑69 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAg > 1:784751/1‑69 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAg > 1:113721/1‑69 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAg > 1:1983368/1‑69 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAg > 1:2248785/1‑69 (MQ=255) gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAg > 1:2272016/1‑69 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:634930/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:2739205/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:433437/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:1523803/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:1582751/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:332486/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:1755/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:2852582/1‑68 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:2393484/1‑68 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc > 1:561868/1‑69 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc > 1:1232214/1‑69 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc > 1:2950396/1‑69 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc > 1:2541471/1‑69 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc > 1:1130720/1‑69 (MQ=255) caTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtcat < 1:1449815/69‑1 (MQ=255) caTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtcat < 1:2753155/69‑1 (MQ=255) | TCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCAT > minE/109630‑109765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |