| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,367,302 | C→T | 32.2% | V47I (GTT→ATT) | yohG ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,367,302 | 0 | C | T | 32.2% | 55.8 / 30.3 | 65 | V47I (GTT→ATT) | yohG | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (20/24); new base T (7/14); total (27/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTG > minE/1367237‑1367369 | cagcAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTc > 1:2571792/1‑69 (MQ=255)cagcAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTc > 1:1999167/1‑69 (MQ=255)cagcAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTc > 1:26417/1‑69 (MQ=255)cagcAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTc > 1:1635688/1‑69 (MQ=255) ggTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCtt > 1:1812491/1‑69 (MQ=255) ggTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCtt > 1:1734511/1‑69 (MQ=255) gTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:2259338/69‑1 (MQ=255) gTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:2348240/69‑1 (MQ=255) gTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:2600200/69‑1 (MQ=255) gTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:315217/69‑1 (MQ=255) gTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:1499588/69‑1 (MQ=255) gTTTGCTCGCGGTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:2481103/69‑1 (MQ=255) tttGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGGGGGCAGTAACCTCCGACCGATTCttt < 1:3362507/68‑1 (MQ=255) tttGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:1775409/68‑1 (MQ=255) tttGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:1929746/68‑1 (MQ=255) tttGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:1958282/68‑1 (MQ=255) tttGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:2107833/68‑1 (MQ=255) tttGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTc > 1:554031/1‑69 (MQ=255) cgcgTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCttt < 1:1515687/62‑1 (MQ=255) ccgccgCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGata > 1:405856/1‑65 (MQ=255) ccgccgCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGata > 1:1424960/1‑65 (MQ=255) ccgccgCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGata > 1:813645/1‑65 (MQ=255) ccgccgCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGata > 1:2586260/1‑65 (MQ=255) ccgccgCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGata > 1:2425580/1‑65 (MQ=255) ccgccgCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGat > 1:1221504/1‑64 (MQ=255) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:11523/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:920786/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:869242/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:428912/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:3260050/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:2202137/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:1507720/52‑1 (MQ=39) cgccgcCCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTc < 1:2312007/52‑1 (MQ=39) ccgccCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGAt < 1:2121679/69‑1 (MQ=255) ccgccCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGAt < 1:136593/69‑1 (MQ=255) ccgccCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGAt < 1:821546/69‑1 (MQ=255) ccgccCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGAt < 1:1698687/69‑1 (MQ=255) ccgccCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGAt < 1:593511/69‑1 (MQ=255) ccgccCGTGCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGAt < 1:1438883/69‑1 (MQ=255) cGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAg < 1:1370024/69‑1 (MQ=255) cGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAg < 1:3211109/69‑1 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGccc > 1:2138473/1‑69 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGccc > 1:919889/1‑69 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGccc > 1:1450361/1‑69 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGccc > 1:1452306/1‑69 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGccc > 1:1454265/1‑69 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGccc > 1:1598978/1‑69 (MQ=255) gCTTTAACCGTACCCAGGCGAGCAGTAATCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGcc > 1:3136771/1‑68 (MQ=255) cTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCccg > 1:1763297/1‑69 (MQ=255) cTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCccg > 1:618003/1‑69 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:3153517/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:1819694/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:2730888/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:801845/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:554823/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:2747803/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:366459/41‑1 (MQ=255) gCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:3165156/41‑1 (MQ=255) gCGGGAAGTAACCTCCGCCCGATTCTTTCCCCAGATATCga < 1:548513/41‑1 (MQ=255) aGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAg < 1:2743447/43‑1 (MQ=255) aaCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTgg > 1:357441/1‑68 (MQ=255) aaCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTgg > 1:1167213/1‑68 (MQ=255) aCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGgtg > 1:295606/1‑69 (MQ=255) aCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGgtg > 1:2157520/1‑69 (MQ=255) aCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGATGgtg > 1:3390105/1‑69 (MQ=255) | CAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTG > minE/1367237‑1367369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |