| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,368,902 | C→T | 59.2% | T171T (ACG→ACA) | dusC ← | tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,368,902 | 0 | C | T | 59.2% | 27.6 / 54.0 | 76 | T171T (ACG→ACA) | dusC | tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base C (19/12); new base T (19/26); total (38/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGATTTCAAATTTC > minE/1368841‑1368968 | gCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGcc < 1:3073873/69‑1 (MQ=255)gCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGcc < 1:2604999/69‑1 (MQ=255) tGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCa < 1:291949/69‑1 (MQ=255) tGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCa < 1:207751/69‑1 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:2032384/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:3007885/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:2957926/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:1482968/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:932076/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:1345688/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTa > 1:959439/1‑69 (MQ=255) gCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGAACCACCAGCTCCGTa > 1:2030884/1‑69 (MQ=255) ccGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCg < 1:1412516/69‑1 (MQ=255) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:1319290/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:3415749/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:1500152/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:2401277/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:2390484/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:3363670/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:1114262/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:179035/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:3011844/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:1107951/1‑34 (MQ=37) cgcgGTAACCCTGCTCTTTTGTCCGCCCATGtact > 1:2832758/1‑34 (MQ=37) gTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:3291015/69‑1 (MQ=255) gTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:1939175/69‑1 (MQ=255) gTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:1548642/69‑1 (MQ=255) gTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:8772/69‑1 (MQ=255) gTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:1528656/69‑1 (MQ=255) tAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:2299130/68‑1 (MQ=255) accccTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTCTTGa > 1:657678/3‑56 (MQ=255) aaCCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGtt > 1:1072347/1‑54 (MQ=255) aaCCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGa > 1:509387/1‑69 (MQ=255) aaCCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGa > 1:117332/1‑69 (MQ=255) aaCCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGa > 1:2649399/1‑69 (MQ=255) aaCCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGa > 1:1209639/1‑69 (MQ=255) aaCCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGa > 1:2567692/1‑69 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTg > 1:3039058/1‑48 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTGTTg > 1:1954068/1‑51 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTGTTg > 1:1048131/1‑51 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTGTTg > 1:2528960/1‑51 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTGTTg > 1:487118/1‑51 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTGTTg > 1:872692/1‑51 (MQ=255) cTGCTCTTTTGTCCGCCCATGTACTACCAGCTCAGTAGCGCCAGCCTGTTg > 1:1183864/1‑51 (MQ=37) cTGCTCTTTTGACCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCTGTTg > 1:2304626/1‑51 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:3186300/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:818697/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:3342846/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:396306/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:622774/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:792338/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2554535/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:831207/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:96520/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2900049/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:1542167/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:1691467/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:1996670/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2110566/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2144840/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2182417/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2246715/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2435593/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:1007077/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2735674/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2777512/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2834841/46‑1 (MQ=255) tGCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:294177/46‑1 (MQ=255) gCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:2429234/45‑1 (MQ=255) gCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:3392677/45‑1 (MQ=255) gCTCTTTTGTCCGCCCATGTACTACCAGCTCCGTAGCGCCAGCCt < 1:1445098/45‑1 (MQ=255) ctctTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGATTTCaaa > 1:774193/1‑69 (MQ=255) ctctTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGATTTCaaa > 1:868111/1‑69 (MQ=255) ctctTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGATTTCaa > 1:1396116/1‑68 (MQ=255) tctTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGc < 1:620100/59‑1 (MQ=255) ttCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGATTTCAAATttc > 1:1012614/1‑69 (MQ=255) | GCTGGCGAATATCGCCAATCGCCTGCCAGTCAATATGCTCCGCGCGGTAACCCTGCTCTTTCGTCCGCCCATGCACCACCAGCTCCGTAGCGCCAGCCTGTTGAACCGCATCGGCGATTTCAAATTTC > minE/1368841‑1368968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |