| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,369,001 | C→T | 52.5% | V138V (GTG→GTA) | dusC ← | tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,369,001 | 0 | C | T | 52.5% | ‑2.1 / 32.6 | 40 | V138V (GTG→GTA) | dusC | tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base C (14/5); new base T (7/14); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
CTGTTGAACCGCATCGGCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTA > minE/1368938‑1369057 | cTGTTGAACCGCATCGGCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCt < 1:2192671/69‑1 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:1126132/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:33743/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:3323892/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:2989804/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:2680265/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:2671501/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:2399060/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:2365599/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:2113442/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:1690178/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:157936/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:1477482/1‑69 (MQ=255) ggCGATTTCAAATTTCTTCTCACAGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcgc > 1:366410/1‑69 (MQ=255) ggCGATTTCAAAATTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATgcg > 1:1858093/1‑68 (MQ=255) ttCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATGCGCCGGTAc < 1:46711/69‑1 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:892749/1‑69 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:86066/1‑69 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:105164/1‑69 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:1130756/1‑69 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:123344/1‑69 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:1514656/1‑69 (MQ=255) agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:1289747/1‑69 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2582926/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2239519/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:876853/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:678009/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:592353/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2543595/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:1283950/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2584032/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:320277/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:3185724/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:3092281/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2919236/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCAGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2982485/69‑1 (MQ=255) cagACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTa < 1:257481/69‑1 (MQ=255) agACGCACTTTTACTCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2792646/68‑1 (MQ=255) agACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTa < 1:477441/68‑1 (MQ=255) agACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTa < 1:502713/68‑1 (MQ=255) | CTGTTGAACCGCATCGGCGATTTCAAATTTCTTCTCACCGCTGTCCCAGCCCAGACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTA > minE/1368938‑1369057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |