| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,369,034 | A→G | 67.9% | R127R (CGT→CGC) | dusC ← | tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,369,034 | 0 | A | G | 67.9% | 50.3 / 27.3 | 59 | R127R (CGT→CGC) | dusC | tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base A (9/10); new base G (26/14); total (35/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
AGCCCAGACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCCCGCCG > minE/1368985‑1369094 | agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:105164/1‑69 (MQ=255)agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:1514656/1‑69 (MQ=255)agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:86066/1‑69 (MQ=255)agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:1289747/1‑69 (MQ=255)agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:892749/1‑69 (MQ=255)agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:123344/1‑69 (MQ=255)agcccagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCt > 1:1130756/1‑69 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:876853/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2543595/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:320277/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2239519/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2582926/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2584032/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:3185724/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2919236/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:592353/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:678009/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:3092281/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:1283950/69‑1 (MQ=255) cagACGCACTTTTACGCTGACAGGCAAATGCGCCAGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2982485/69‑1 (MQ=255) cagACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTa < 1:257481/69‑1 (MQ=255) agACGCACTTTTACTCTGACAGGCAAATGCGCCGGTACAGCTTCGCGCATCGCTTTTGCACCCTGGTa < 1:2792646/68‑1 (MQ=255) agACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTa < 1:502713/68‑1 (MQ=255) agACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTa < 1:477441/68‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:298602/48‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:3139921/48‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:2062419/48‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:1906090/48‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:1493310/48‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:1450205/48‑1 (MQ=255) aCGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTgg < 1:1431246/48‑1 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:3271070/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:3344022/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:3200418/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:349819/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:3179600/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:480773/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1112175/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:2932095/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1234415/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1458404/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1523044/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1659943/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1683271/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:1722110/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:2045253/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:2236776/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacca > 1:2750661/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacc > 1:2345718/1‑68 (MQ=255) aCAGCTTCGCGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTCAGTAACGTTGCCccacc > 1:3154599/1‑68 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCcctccg > 1:3270951/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgccg > 1:2246331/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgccg > 1:340112/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgccg > 1:2590416/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgccg > 1:1347403/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgccg > 1:880216/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgccg > 1:2954874/1‑69 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgcc > 1:2820399/1‑68 (MQ=255) aCAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCccgcc > 1:1259940/1‑68 (MQ=255) | AGCCCAGACGCACTTTCACGCTGACGGGCAAATGCGCCGGTACAGCTTCACGCATCGCTTTTGCACCCTGGTAGATGAGTTCAGGATCTTTGAGTAACGTCGCCCCGCCG > minE/1368985‑1369094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |