Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,371,806	C→T	100%	intergenic (+49/‑101)	cdd → / → sanA	cytidine/deoxycytidine deaminase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,371,806	0	C	T	93.1%	23.8 / ‑4.7	14	intergenic (+49/‑101)	cdd/sanA	cytidine/deoxycytidine deaminase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (13/0);  total (14/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGT  >  minE/1371793‑1371869              |                                                                gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGc                                           >  1:3430583/1‑36 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:1228289/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:1616517/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:1644643/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:2169148/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:2170801/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:2223302/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:2474912/1‑37 (MQ=37) gCTTGCCAGCCCTTTCCTGGTGTCGAAAATCCCGGCa                                          >  1:2993438/1‑37 (MQ=37)        aGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTaa     >  1:1164719/1‑67 (MQ=255)         gaccTTTCCTGGTGTCGAAAATCCCGGCAAACAGTTTGCCGttt                           >  1:3150603/3‑44 (MQ=37)         gCCCTTTCCTGGTGTCGAAAATCCCGGCAAACAGTTTGCCGTTTCTTGCTCAAAACCAGCGGGTAAAGt  >  1:936434/1‑69 (MQ=255)         gCCCTTTCCTGGTGTCGAAAATCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGt  >  1:3406092/1‑69 (MQ=255)         gCCCTTTCCTGGTGTCGAAAATCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGt  >  1:96089/1‑69 (MQ=255)              |                                                                GCTTGCCAGCCCTCTCCTGGTGTCGAAATTCCCGGCAAACAGTTTGCCGTTTCTTGCGCAAAACCAGCGGGTAAAGT  >  minE/1371793‑1371869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑