| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,377,077 | C→T | 32.2% | K414K (AAG→AAA) | mglA ← | fused methyl‑galactoside transporter subunitsl and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,377,077 | 0 | C | T | 32.2% | 57.7 / 32.7 | 68 | K414K (AAG→AAA) | mglA | fused methyl‑galactoside transporter subunitsl and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (11/35); new base T (18/4); total (29/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
CCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCG > minE/1377012‑1377144 | ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:979615/69‑1 (MQ=255)ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:2103738/69‑1 (MQ=255)ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:463787/69‑1 (MQ=255)ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:191805/69‑1 (MQ=255)ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:2297753/69‑1 (MQ=255)ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:232550/69‑1 (MQ=255)ccGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:2531446/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:1627209/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:3265945/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:3062594/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:1909583/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:3048495/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:2830221/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:1986890/69‑1 (MQ=255) gcgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:457614/69‑1 (MQ=255) cgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:2193690/68‑1 (MQ=255) cgTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTg > 1:569497/1‑69 (MQ=255) gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCttt < 1:2266020/63‑1 (MQ=255) gTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:997064/67‑1 (MQ=255) tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAt < 1:2740763/69‑1 (MQ=255) tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAt < 1:2692987/69‑1 (MQ=255) tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAt < 1:994029/69‑1 (MQ=255) tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAt < 1:1716496/69‑1 (MQ=255) tCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAt < 1:2491952/69‑1 (MQ=255) cGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAt < 1:2090635/68‑1 (MQ=255) ggTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCtttt > 1:659290/1‑61 (MQ=255) ggTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAtt > 1:456719/1‑68 (MQ=255) ggTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAtt > 1:2202529/1‑68 (MQ=255) ggTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAtt > 1:2223942/1‑68 (MQ=255) ggTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAtt > 1:96956/1‑68 (MQ=255) ggTTCATCGAGCATTAATATTTATGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGAtt > 1:3377670/1‑68 (MQ=255) tCATCGAGCATTAATATTTCTGGTTGCGTTAACAGCCAGCGACCAATAATCACTTTTTGCTGATTacca < 1:1816455/69‑1 (MQ=39) tCATCGAGCATTAATATTTCTGGTTGCGTTAACAGCCAGCGACCAATAATCACTTTTTGCTGATTacca < 1:1073221/69‑1 (MQ=39) tCATCGAGCATTAATATTTCTGGTTGCGTTAACAGCCAGCGACCAATAATCACTTTTTGCTGATTacca < 1:751084/69‑1 (MQ=39) cATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:780338/60‑1 (MQ=255) aTTAATATTTCTGGTTGCGTTCACAGCCAGCGACCAATAATCACTTTTTGCTGATTacca < 1:7612/60‑1 (MQ=37) atatTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCt < 1:540387/48‑1 (MQ=255) tttCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCaa < 1:3068344/69‑1 (MQ=255) tttCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCaa < 1:2181225/69‑1 (MQ=255) ggTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCaa < 1:2446003/64‑1 (MQ=255) gCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTcc < 1:558535/60‑1 (MQ=255) gCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTcc < 1:939854/60‑1 (MQ=255) gCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTcc < 1:2746577/60‑1 (MQ=255) gCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTcc < 1:1360392/60‑1 (MQ=255) gCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTcc < 1:137077/60‑1 (MQ=255) gCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTcc < 1:1936562/60‑1 (MQ=255) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGga > 1:1879010/1‑38 (MQ=255) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:2827723/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:898844/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:882816/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:822365/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:1506733/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:151383/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:1653042/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:1733686/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:1759104/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:207090/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:2082690/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:2330090/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:2513362/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:3253683/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:3174598/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:271721/1‑68 (MQ=38) cAGCGACCAATAATCACTTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGCCCCggt > 1:3046076/1‑68 (MQ=38) atCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGc > 1:2640530/1‑69 (MQ=255) atCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGc > 1:718668/1‑69 (MQ=255) atCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGc > 1:2971597/1‑69 (MQ=255) atCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGc > 1:1359244/1‑69 (MQ=255) cTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCg < 1:2325159/68‑1 (MQ=255) | CCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATCG > minE/1377012‑1377144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |