| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 110,575 | G→A | 55.3% | P47P (CCG→CCA) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 110,575 | 0 | G | A | 55.3% | 5.5 / 36.0 | 47 | P47P (CCG→CCA) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (8/13); new base A (16/10); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
CGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGC > minE/110509‑110640 | cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:1013154/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:918112/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:914082/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:819563/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:73921/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:683246/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:554147/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:3031856/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:2725584/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:2663022/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:2019370/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:1938202/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:173519/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:1415393/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:1401380/1‑69 (MQ=255)cGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTACCAtc > 1:1185292/1‑69 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTa > 1:1490026/1‑66 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTa > 1:1301574/1‑66 (MQ=255) cGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTa > 1:3023321/1‑66 (MQ=255) aCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCaaa > 1:963238/1‑69 (MQ=255) aCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCaaa > 1:1125461/1‑69 (MQ=255) aCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCaaa > 1:3340893/1‑69 (MQ=255) aGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGtctc < 1:3414991/69‑1 (MQ=255) gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTctct < 1:1663411/69‑1 (MQ=255) gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTctct < 1:2557225/69‑1 (MQ=255) gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTctct < 1:854161/69‑1 (MQ=255) gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTctct < 1:3285055/69‑1 (MQ=255) gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTctct < 1:1216884/69‑1 (MQ=255) gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTctct < 1:413188/69‑1 (MQ=255) tctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc < 1:577087/69‑1 (MQ=255) tctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc < 1:2263724/69‑1 (MQ=255) tctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc < 1:119481/69‑1 (MQ=255) tctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc < 1:1312221/69‑1 (MQ=255) tctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc < 1:3078005/69‑1 (MQ=255) tctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAcc < 1:3014741/69‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:525385/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:1258319/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:474318/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:673307/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:6950/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:2528587/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:786945/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:233784/67‑1 (MQ=255) gAAGTACCATCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:1329964/67‑1 (MQ=255) gAAGTACCATCTCCGAAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 1:100659/67‑1 (MQ=255) tCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGgcgc > 1:886005/1‑69 (MQ=255) tCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGgcg > 1:1523150/1‑68 (MQ=255) | CGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGC > minE/110509‑110640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |