| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,381,097 | C→T | 24.2% | A300A (GCG→GCA) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,381,097 | 0 | C | T | 24.2% | 49.6 / 10.5 | 41 | A300A (GCG→GCA) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (9/22); new base T (4/6); total (13/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCA > minE/1381031‑1381160 | aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:95189/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:2554425/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:232939/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:2964201/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:3209890/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:404340/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:188631/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:1683343/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:1446894/69‑1 (MQ=255)aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:1158188/69‑1 (MQ=255) gCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa > 1:590054/1‑68 (MQ=255) gCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa > 1:923349/1‑68 (MQ=255) cAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAg > 1:1341603/1‑68 (MQ=255) cAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAg > 1:2109197/1‑68 (MQ=255) aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:742659/69‑1 (MQ=255) aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:3423196/69‑1 (MQ=255) aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:1130118/69‑1 (MQ=255) aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:1080308/69‑1 (MQ=255) aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:2213161/69‑1 (MQ=255) aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:171347/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:2338805/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:987612/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:860887/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:707166/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:619192/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:593365/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:3244820/69‑1 (MQ=255) tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:3232771/69‑1 (MQ=255) ttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:1271162/68‑1 (MQ=255) agatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:387978/1‑69 (MQ=255) agatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:1928512/1‑69 (MQ=255) agatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:2345604/1‑69 (MQ=255) agatagGTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:440677/1‑69 (MQ=255) ttGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:2702866/55‑1 (MQ=255) cAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGc < 1:364233/68‑1 (MQ=255) aGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGc < 1:778631/67‑1 (MQ=255) cgtccgACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGc > 1:378523/1‑69 (MQ=255) gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:1988465/1‑69 (MQ=255) gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:2047514/1‑69 (MQ=255) gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:1480871/1‑69 (MQ=255) gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:2981246/1‑69 (MQ=255) | AAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCA > minE/1381031‑1381160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |