| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,381,217 | G→A | 36.3% | R260R (CGC→CGT) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,381,217 | 0 | G | A | 36.3% | 58.2 / 51.5 | 88 | R260R (CGC→CGT) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (39/17); new base A (25/7); total (64/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGC > minE/1381150‑1381282 | aCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGc < 1:1924736/69‑1 (MQ=255) gCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGa < 1:1891173/69‑1 (MQ=255) gCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGa < 1:691064/69‑1 (MQ=255) gCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGa < 1:1786188/69‑1 (MQ=255) gCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGa < 1:2814068/69‑1 (MQ=255) gCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGa < 1:1613039/69‑1 (MQ=255) gCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTc > 1:2885475/1‑68 (MQ=255) tGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGc > 1:390244/1‑69 (MQ=255) tGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGc > 1:1590856/1‑69 (MQ=255) aCGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGc < 1:2624842/68‑1 (MQ=255) aCGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGc < 1:798999/68‑1 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGcc > 1:1523767/1‑46 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:2142104/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:1869898/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:2976851/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:1604356/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:3054317/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:3180118/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:523860/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:59738/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:757852/1‑69 (MQ=255) cAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:88766/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCa > 1:2742408/1‑49 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGa > 1:2562658/1‑55 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt > 1:162780/1‑67 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:3061077/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:30670/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:3344680/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:2848787/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:690024/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:742017/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:1072202/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:1690378/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:1575903/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:2001226/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:1962945/1‑69 (MQ=255) tGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa > 1:1336427/1‑69 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCTCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1203362/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAAt > 1:781647/1‑67 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATga > 1:1781436/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:3003812/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2881144/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1621589/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:366929/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2929818/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1617971/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1452953/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:3045604/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1554630/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1550628/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:3190085/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:3248927/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2871750/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2868557/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1341443/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2585134/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1318021/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1296879/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2516296/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:1225857/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:2306538/1‑68 (MQ=255) gTAAGAAGGCGCACCAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATg > 1:900950/1‑68 (MQ=255) agaagGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGt > 1:2614647/1‑60 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:434046/1‑41 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:3427442/1‑41 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:3378416/1‑41 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:908738/1‑41 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:1995028/1‑41 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGc > 1:2528512/1‑41 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:2072828/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:2918326/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:2801926/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:1104130/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:561199/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:1196914/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:2525011/56‑1 (MQ=255) gCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:2458756/56‑1 (MQ=255) cacaCCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt < 1:451868/55‑1 (MQ=255) ccAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGc > 1:3415949/1‑69 (MQ=255) ccAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGc > 1:1050115/1‑69 (MQ=255) ccAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGc > 1:270530/1‑69 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:2479137/69‑1 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:689715/69‑1 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:2244152/69‑1 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:851235/69‑1 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:1526395/69‑1 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:2237669/69‑1 (MQ=255) ccAACGATACGCCCAGTCCAGTCGCCATTGCAGGGCGATGGCAGGAAGGTTAATGATCACCCCAATCGc < 1:3322341/69‑1 (MQ=255) | ACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGC > minE/1381150‑1381282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |