| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,381,675 | A→G | 55.6% | L108L (TTG→CTG) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,381,675 | 0 | A | G | 55.6% | 3.8 / 26.6 | 36 | L108L (TTG→CTG) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (4/12); new base G (14/6); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAC > minE/1381607‑1381742 | gCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCaa < 1:1147437/69‑1 (MQ=255) gATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGcc < 1:780150/68‑1 (MQ=255) gATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGcc < 1:3127115/68‑1 (MQ=255) gATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGcc < 1:298354/68‑1 (MQ=255) gcgcACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAAc > 1:1454478/1‑67 (MQ=255) gcgcACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACa > 1:1754269/1‑68 (MQ=255) aaGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCca > 1:2578813/1‑67 (MQ=255) aaGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCca > 1:174498/1‑67 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa < 1:1238482/68‑1 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa < 1:2854311/68‑1 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:3372201/69‑1 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:2131435/69‑1 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:2771183/69‑1 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:3406825/69‑1 (MQ=255) cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:680495/69‑1 (MQ=255) accaGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACt < 1:617227/69‑1 (MQ=255) accaGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACt < 1:2354007/69‑1 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2526983/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2255182/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:290880/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:785878/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:797832/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1631546/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1149027/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:134007/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1381123/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1481970/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2515294/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1828850/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:239462/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAAAAAAGCCCAGCAAGACt > 1:672072/1‑68 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCTTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1428743/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:164796/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1572003/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1840454/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1857597/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1175429/69‑1 (MQ=255) aaaaTATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAc < 1:2958829/69‑1 (MQ=255) aaaaTATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAAAAAGACTAACAGCGTTAACCGCGAc < 1:1659355/69‑1 (MQ=255) | GCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAC > minE/1381607‑1381742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |