| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,381,717 | A→G | 60.7% | L94L (TTG→CTG) | yeiB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,381,717 | 0 | A | G | 60.7% | 31.8 / 41.8 | 66 | L94L (TTG→CTG) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (16/10); new base G (33/7); total (49/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTGC > minE/1381653‑1381778 | cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa < 1:1238482/68‑1 (MQ=255)cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa < 1:2854311/68‑1 (MQ=255)cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:3406825/69‑1 (MQ=255)cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:2131435/69‑1 (MQ=255)cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:2771183/69‑1 (MQ=255)cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:3372201/69‑1 (MQ=255)cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:680495/69‑1 (MQ=255) accaGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACt < 1:2354007/69‑1 (MQ=255) accaGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACt < 1:617227/69‑1 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1631546/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1149027/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1828850/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2255182/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:239462/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2515294/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2526983/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:797832/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:785878/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:290880/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:134007/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1481970/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1381123/1‑68 (MQ=255) ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAAAAAAGCCCAGCAAGACt > 1:672072/1‑68 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCTTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1428743/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1175429/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1857597/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1572003/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1840454/69‑1 (MQ=255) aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:164796/69‑1 (MQ=255) aaaaTATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAc < 1:2958829/69‑1 (MQ=255) aaaaTATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAAAAAGACTAACAGCGTTAACCGCGAc < 1:1659355/69‑1 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:2744114/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:3196078/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:2865062/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:547202/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:572082/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:2360273/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa > 1:1397010/1‑44 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAACCCAACAAGACTAACAGCGTTAACCGCGACTgg > 1:148622/1‑69 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAACCCAACAAGACTAACAGCGTTAACCGCGACTgg > 1:36505/1‑69 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAACCCAACAAGACTAACAGCGTTAACCGCGACTgg > 1:1691711/1‑69 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAACCCAACAAGACTAACAGCGTTAACCGCGACTgg > 1:1296172/1‑69 (MQ=255) atatCGCCGTCCCAGAACAATAAACCGTGAATAAAACCCAACAAGACTAACAGCGTTAACCGCGACTgg > 1:2950760/1‑69 (MQ=255) atatCGCCGTACCAGAACAATAAACCGTGAATAAAACCCAACAAGACTAACAGCGTTAACCGCGACTgg > 1:2954671/1‑69 (MQ=255) tatCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCTGCAAGACTAACAGCGTTa < 1:2797595/57‑1 (MQ=255) cGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGc < 1:3053499/69‑1 (MQ=255) aCAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCAc > 1:2384243/1‑69 (MQ=255) aCAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCAc > 1:2598658/1‑69 (MQ=255) cAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACg > 1:3019563/1‑69 (MQ=255) cAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACg > 1:1785503/1‑69 (MQ=255) cAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACg > 1:1663686/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATc > 1:3429320/1‑46 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:1283483/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:1542241/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:350110/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:74145/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:1354791/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:426746/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:915816/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:3319865/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:3278849/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:3036316/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:3017932/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:272546/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:2532414/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:16710/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:1779160/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:2124205/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCACGGGGCAGCaa > 1:1932986/1‑69 (MQ=255) gTGAATAAAGCCCAGCAAGACTAACAGCGTTAACCGCGACTGGATCCATCGTCTGCCAAGGGGCAGCaa > 1:2855085/1‑69 (MQ=255) aaGCCCAACAAGACTAACAGCGTTTACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTGc > 1:2058723/1‑69 (MQ=255) aaGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTg > 1:156589/1‑68 (MQ=255) aaGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTGc > 1:2244887/1‑69 (MQ=255) aaGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTGc > 1:718143/1‑69 (MQ=255) aaGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTGc > 1:2120134/1‑69 (MQ=255) | CCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATCCAGCGTCTGCCACGGGGCAGCAACATTTGC > minE/1381653‑1381778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |