| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,390,648 | C→T | 36.6% | L260L (CTC→CTT) | nfo → | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,390,648 | 0 | C | T | 36.6% | 58.2 / 53.4 | 90 | L260L (CTC→CTT) | nfo | endonuclease IV with intrinsic 3'‑5' exonuclease activity |
| Reads supporting (aligned to +/- strand): ref base C (24/33); new base T (14/19); total (38/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
TATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAA > minE/1390582‑1390705 | tatCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGGATTCCGCTGATCCTCGa < 1:3178853/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:1720886/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:87606/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:2352895/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:2375357/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:2093202/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:206836/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:2518815/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:1947427/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:2667012/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:1820829/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:1775744/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:3099730/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:1403373/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:3425898/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:3177025/69‑1 (MQ=255) atCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGa < 1:3111002/68‑1 (MQ=255) atCCGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:417422/69‑1 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa > 1:2997190/1‑68 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa > 1:2461240/1‑68 (MQ=255) tCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGaa > 1:1985528/1‑68 (MQ=255) gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:1956191/69‑1 (MQ=255) gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:320417/69‑1 (MQ=255) gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:1203382/69‑1 (MQ=255) gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:2924243/69‑1 (MQ=255) gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:1928741/69‑1 (MQ=255) gCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:1735084/69‑1 (MQ=255) gCGTTCAGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCg < 1:1133912/69‑1 (MQ=255) cGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCAGATCCTCGAAACCATCAACCCg > 1:2917577/1‑68 (MQ=255) tCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:2048927/53‑1 (MQ=255) ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat > 1:2716616/1‑69 (MQ=255) ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat > 1:1200291/1‑69 (MQ=255) ccGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGatat > 1:1647533/1‑69 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:492224/68‑1 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:3129091/68‑1 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:776025/68‑1 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:777913/68‑1 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:1356256/68‑1 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:1936034/68‑1 (MQ=255) gCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATc < 1:1467917/68‑1 (MQ=255) tCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGc > 1:832425/1‑67 (MQ=255) aTGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:856396/42‑1 (MQ=255) ctaGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:46833/67‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTTGaa < 1:810947/39‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:2303453/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:1031683/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:1002243/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:3256440/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:985693/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:975239/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:2281445/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:559389/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:890314/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:1895662/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:247643/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:2060035/69‑1 (MQ=255) cAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:2149466/69‑1 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCa > 1:2700974/1‑42 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATc > 1:1192603/1‑68 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:1622850/1‑69 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:477550/1‑69 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:1275796/1‑69 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:2126590/1‑69 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:428858/1‑69 (MQ=255) acgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCg > 1:1062696/1‑69 (MQ=255) cGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:422347/60‑1 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGatat > 1:1418956/1‑43 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:678145/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:763742/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:971665/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:778409/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:88409/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:991324/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:126468/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:2924747/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:2499878/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:2416738/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:2240032/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCt > 1:1998527/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTTGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCACCTGGCt > 1:3363824/1‑67 (MQ=255) tCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGa < 1:2516321/69‑1 (MQ=255) gCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAgcgc > 1:2850813/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTTGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:1292515/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:1138284/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:443864/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:1571974/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:1807673/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:3406712/1‑69 (MQ=255) ccGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGcaacaa > 1:379212/1‑69 (MQ=255) gATCCTCGAAACCATCAACCCGGATATCTGGGCAGAagag < 1:984449/40‑1 (MQ=255) | TATCGGTCATGATGCGTTCCGCTGGATCATGCAAGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAA > minE/1390582‑1390705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |