Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 111,038 C→T 35.8% P202S (CCA→TCA)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE111,0380CT35.8% 23.9 / 18.9 39P202S (CCA→TCA) aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base C (15/10);  new base T (2/12);  total (17/22)
Fisher's exact test for biased strand distribution p-value = 7.77e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGT  >  minE/110981‑111105
                                                         |                                                                   
gCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTgg                                                           <  1:459906/68‑1 (MQ=255)
gCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTgg                                                           <  1:3096628/68‑1 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTgg                                                           >  1:1841440/1‑63 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCg                                                         >  1:881340/1‑65 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGt                                                        >  1:1132958/1‑66 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGt                                                        >  1:176621/1‑66 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGt                                                        >  1:2383850/1‑66 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGt                                                        >  1:2822082/1‑66 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGt                                                        >  1:3128554/1‑66 (MQ=255)
     cgcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCACTGCACCAGCGGCTGGCGt                                                        >  1:2058129/1‑66 (MQ=255)
     cgcgGCAGCTCCGGACGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGt                                                        >  1:3006662/1‑66 (MQ=255)
      gcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGaa                                                     >  1:2287845/1‑68 (MQ=255)
      gcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGaa                                                     >  1:3319047/1‑68 (MQ=255)
      gcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGaa                                                     >  1:2126058/1‑68 (MQ=255)
      gcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGaa                                                     >  1:2146041/1‑68 (MQ=255)
      gcgGCAGCTCCGGCCGCTAAACAGGAAGCAGCGCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGaa                                                     >  1:2680358/1‑68 (MQ=255)
            gCTCCGGCGGCTAAACAGGAAGCGGCTCCGGCGGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGtt                                              >  1:199108/1‑69 (MQ=255)
            gCTCCGGCGGCTAAACAGGAAGCGGCTCCGGCAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGtt                                              >  1:252189/1‑69 (MQ=255)
                  gCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGtt                                        <  1:193003/69‑1 (MQ=255)
                  gCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGtt                                        <  1:3230400/69‑1 (MQ=255)
                  gCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGtt                                        <  1:1767932/69‑1 (MQ=255)
                   ccGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGtt                                        <  1:124064/68‑1 (MQ=255)
                              gAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCAGGATATcggc                            >  1:1349981/1‑69 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:133538/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:2393615/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:860000/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:537316/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:2202199/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:1978538/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:189582/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:2396482/69‑1 (MQ=255)
                                           cAGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:3407571/69‑1 (MQ=255)
                                            aGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:1856447/68‑1 (MQ=255)
                                            aGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:42097/68‑1 (MQ=255)
                                            aGCGGCCCCTGCATCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGTGGTGACGAAGTTg               <  1:340505/68‑1 (MQ=255)
                                               ggCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGt           <  1:3286317/69‑1 (MQ=255)
                                                gCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTg          <  1:2118296/69‑1 (MQ=255)
                                                gCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTg          <  1:83258/69‑1 (MQ=255)
                                                  cccTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGAc        <  1:520259/69‑1 (MQ=255)
                                                         ccAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGt  <  1:2319990/68‑1 (MQ=255)
                                                         ccAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGt  <  1:2467755/68‑1 (MQ=255)
                                                         ccAGCGGCTGGCGTGAAAGAAGTTAACGTTACGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGt  <  1:2248332/68‑1 (MQ=255)
                                                         |                                                                   
GCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAGCAGCTCCGGCAGCGGCCCCTGCACCAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGT  >  minE/110981‑111105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: