| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,398,615 | T→C | 44.2% | H313H (CAT→CAC) | yeiQ → | predicted dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,398,615 | 0 | T | C | 44.2% | 16.4 / 68.8 | 86 | H313H (CAT→CAC) | yeiQ | predicted dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (32/16); new base C (19/19); total (51/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
GCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCA > minE/1398552‑1398680 | gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:113331/64‑1 (MQ=255)gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:333630/64‑1 (MQ=255)gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:2505649/64‑1 (MQ=255)gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:2218798/64‑1 (MQ=255)gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:2019758/64‑1 (MQ=255)gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:1997843/64‑1 (MQ=255)gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:1784798/64‑1 (MQ=255) cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAg > 1:1832779/1‑68 (MQ=255) cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt > 1:998017/1‑69 (MQ=255) cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt > 1:2100629/1‑69 (MQ=255) gCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg > 1:2759817/1‑69 (MQ=255) gCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg > 1:1875120/1‑69 (MQ=255) cTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgc > 1:1180177/1‑69 (MQ=255) cTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgc > 1:1809544/1‑69 (MQ=255) ggCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAg > 1:1491423/1‑69 (MQ=255) gCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg > 1:2344654/1‑69 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2236414/64‑1 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2402129/64‑1 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2151073/64‑1 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:958098/64‑1 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:1867407/64‑1 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2832070/64‑1 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:3125898/1‑69 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:1946379/1‑69 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:2098273/1‑69 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:1442945/1‑69 (MQ=255) aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:370002/1‑69 (MQ=255) tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:1729467/69‑1 (MQ=255) tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:1455869/69‑1 (MQ=255) tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:2186087/69‑1 (MQ=255) tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:594646/69‑1 (MQ=255) tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:489452/69‑1 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg > 1:2084599/1‑60 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg > 1:544732/1‑60 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa > 1:420853/1‑68 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa > 1:2085428/1‑68 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa > 1:1523435/1‑68 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:3080506/1‑69 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:2378833/1‑69 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:3125950/1‑69 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:3431721/1‑69 (MQ=255) ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCAAATATAAGTGATTTCATGCAGGAt > 1:2519385/1‑62 (MQ=255) ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg < 1:2416575/51‑1 (MQ=255) ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg < 1:455417/51‑1 (MQ=255) ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg < 1:853879/51‑1 (MQ=255) ctcGCTTATCTGGATTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGc > 1:1644078/1‑69 (MQ=255) gCTTATCTGGGTTACCTCTCAGGATTCGCCCGTATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:2576403/1‑69 (MQ=255) gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc > 1:3131470/1‑49 (MQ=255) gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:250641/1‑69 (MQ=255) gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:1509649/1‑69 (MQ=255) gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:3043623/1‑69 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:157781/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1759068/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:473066/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1384293/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1832000/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:3124963/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1506074/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:543894/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2795564/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2793598/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2362178/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2776687/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:455282/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1428243/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2202564/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1504768/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2036263/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:717871/1‑61 (MQ=255) tATCTGGGTTACCTCTCAGGATTCGAGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:422101/1‑61 (MQ=255) ctctCAGGATTCGCCCATATCGGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCATAACAtt < 1:564195/68‑1 (MQ=255) ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:779559/68‑1 (MQ=255) ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:1572922/68‑1 (MQ=255) ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:3024160/68‑1 (MQ=255) ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:2961133/68‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:1643792/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:82093/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:688266/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:604331/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:3408188/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2863524/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2745621/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2366060/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2335380/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2164674/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:1461994/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2021892/61‑1 (MQ=255) ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:1859470/61‑1 (MQ=255) aGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGc > 1:2454382/1‑68 (MQ=255) tCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATg > 1:1911713/1‑69 (MQ=255) ccATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCa < 1:3005471/69‑1 (MQ=255) cATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCa < 1:2803614/68‑1 (MQ=255) cATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCa < 1:1262849/68‑1 (MQ=255) | GCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCA > minE/1398552‑1398680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |