| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,400,543 | T→A | 54.6% | P86P (CCT→CCA) | yeiU → | undecaprenyl pyrophosphate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,400,543 | 0 | T | A | 54.6% | 12.8 / 71.8 | 66 | P86P (CCT→CCA) | yeiU | undecaprenyl pyrophosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (26/4); new base A (21/15); total (47/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
TGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAA > minE/1400484‑1400601 | tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga > 1:813228/1‑69 (MQ=255)tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga > 1:446329/1‑69 (MQ=255)tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga > 1:34814/1‑69 (MQ=255)tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga > 1:3232775/1‑69 (MQ=255)tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga > 1:1730065/1‑69 (MQ=255)tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacga > 1:1648086/1‑69 (MQ=255)tgttCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAgacg > 1:2924579/1‑68 (MQ=255) tgctgGGGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc > 1:238126/1‑68 (MQ=255) tgctgGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc > 1:1353115/1‑68 (MQ=255) tgctgGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATc > 1:1762548/1‑68 (MQ=255) ggCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTg < 1:3059179/67‑1 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:991979/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:2830177/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:3018066/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:376333/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:1445183/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:463663/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:556782/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctg > 1:901404/1‑69 (MQ=255) aTGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtct > 1:223389/1‑68 (MQ=255) gTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATtggtctggt > 1:3186283/1‑69 (MQ=255) ttGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGat > 1:2651483/1‑56 (MQ=255) gAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTAtt < 1:1221278/69‑1 (MQ=255) gAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTAtt < 1:255399/69‑1 (MQ=255) tttCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAAc > 1:3108491/1‑69 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTtattat < 1:1438834/44‑1 (MQ=38) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:1596360/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:1087712/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:1494453/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:3422718/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:152932/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:3003860/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:2928773/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:1949802/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:2738290/44‑1 (MQ=255) tGGCTGAAAGAAAACGCCCCAGGCAGACGACGTATCGTGattat < 1:1047542/44‑1 (MQ=255) gaaagaaaACGCCCCAGGCAGACGACGTATCGTGattat < 1:2723192/39‑1 (MQ=255) aaagaaaACGCCCCAGGCAGACGACGTATCGTGattat < 1:1740368/38‑1 (MQ=255) gaaaACGCCCCTGGCAGACGACGTATCGTGATTATtggtctggt < 1:2263585/44‑1 (MQ=255) gaaaACGCCCCAGGCAGCCGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:318812/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGattat < 1:979338/35‑1 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGattat < 1:886631/35‑1 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGTTCATGCTATTAACTGCAGTGGTAt > 1:1788535/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2171357/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2429666/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:456437/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:1286101/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:463730/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:588307/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2304721/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:839511/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:1136256/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:1468376/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2410227/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:3362627/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2197302/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2488713/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2563268/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2599959/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:283202/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTAt > 1:2106642/1‑67 (MQ=255) gaaaACGCCCCAGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGAAt > 1:125169/1‑67 (MQ=255) aaCGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTaa > 1:3373381/1‑67 (MQ=255) aaCGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTaa > 1:3260770/1‑67 (MQ=255) aaCGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTaa > 1:60571/1‑67 (MQ=255) aaCGACCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTaa > 1:2459089/1‑67 (MQ=255) | TGTTCACTGCTGGCGATGGGTATGTTGATGCTGAGTTTCTGGCTGAAAGAAAACGCCCCTGGCAGACGACGTATCGTGATTATTGGTCTGGTCATGCTATTAACTGCAGTGGTATTAA > minE/1400484‑1400601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |