| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,402,436 | C→T | 46.9% | V93V (GTC→GTT) | rtn → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,402,436 | 0 | C | T | 46.9% | 9.2 / 67.6 | 81 | V93V (GTC→GTT) | rtn | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (17/26); new base T (9/29); total (26/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
GACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGA > minE/1402370‑1402498 | gACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCg < 1:1119170/69‑1 (MQ=255)gACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCg < 1:3088627/69‑1 (MQ=255)gACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCg < 1:1281992/69‑1 (MQ=255)gACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCg < 1:3393751/69‑1 (MQ=255) cAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGt > 1:3354490/1‑65 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATTAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1710428/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2332472/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2019109/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1994782/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2409450/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2891160/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1890875/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1859630/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:3284109/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:495064/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1385246/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:500187/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1199642/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:893066/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1054084/69‑1 (MQ=255) gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:975863/69‑1 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:828341/68‑1 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:3326619/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:691099/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:2924645/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:287666/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACATTTGTGCTGGTGAAAGAt > 1:240433/1‑68 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaa > 1:892000/1‑69 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaa > 1:993020/1‑69 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCAGAACGTTTGTGCTGGTGaaa > 1:1037802/1‑61 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATCAATGTCAGa > 1:2019425/1‑43 (MQ=38) ccccGAACTGACCGCCCGCGCAGCGTTTAGAATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaa > 1:1202317/1‑68 (MQ=255) ccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaaaa < 1:2298991/69‑1 (MQ=255) cGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaaaa < 1:3196101/68‑1 (MQ=255) gAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACa < 1:682522/69‑1 (MQ=255) gAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACa < 1:2700279/69‑1 (MQ=255) gAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACa < 1:2618239/69‑1 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:1589031/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:1893460/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:325034/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:1933019/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:2792898/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:954643/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:462052/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:2370547/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAAGGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGt > 1:1118514/1‑66 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1883677/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1580699/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:849138/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:495180/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1351875/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:3158319/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1913613/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:225046/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:2577835/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:2305788/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1998273/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1021748/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:576454/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1039396/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:730440/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:37445/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:2432136/68‑1 (MQ=255) aTGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1070986/67‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1937277/67‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:4584/69‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:1147478/69‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCCGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:1810472/69‑1 (MQ=255) tGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg > 1:875938/1‑68 (MQ=255) gAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGa < 1:3404751/69‑1 (MQ=255) gAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGa < 1:2506770/69‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAATATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:403367/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:332086/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:552252/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:1499896/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:467215/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:1591984/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:2154108/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:2958388/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:2554983/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:2165478/1‑62 (MQ=255) ccGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:2572001/61‑1 (MQ=255) | GACCTTAGATACCTGCCAGCAAGCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGA > minE/1402370‑1402498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |