| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,402,454 | G→A | 40.3% | V99V (GTG→GTA) | rtn → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,402,454 | 0 | G | A | 40.3% | 43.4 / 64.0 | 94 | V99V (GTG→GTA) | rtn | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/31); new base A (9/29); total (34/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCAA > minE/1402392‑1402510 | gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATTAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1710428/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:3284109/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1994782/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1890875/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2019109/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1859630/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2332472/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2409450/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:2891160/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1385246/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:495064/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1199642/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:500187/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:893066/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:1054084/69‑1 (MQ=255)gCTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:975863/69‑1 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGAt < 1:828341/68‑1 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:3326619/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:2924645/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:287666/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGAt > 1:691099/1‑68 (MQ=255) cTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACATTTGTGCTGGTGAAAGAt > 1:240433/1‑68 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaa > 1:892000/1‑69 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaa > 1:993020/1‑69 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCAGAACGTTTGTGCTGGTGaaa > 1:1037802/1‑61 (MQ=255) ccccGAACTGACCGCCCGCGCAGCGTTTAGAATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaa > 1:1202317/1‑68 (MQ=255) ccGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaaaa < 1:2298991/69‑1 (MQ=255) cGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATaaaaaaa < 1:3196101/68‑1 (MQ=255) gAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACa < 1:2618239/69‑1 (MQ=255) gAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACa < 1:2700279/69‑1 (MQ=255) gAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACa < 1:682522/69‑1 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:1933019/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:1589031/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:2792898/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:2370547/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:325034/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:462052/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:1893460/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCa > 1:954643/1‑69 (MQ=255) gcccgcGCGGCGTTTAGCATGAAGGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGt > 1:1118514/1‑66 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:495180/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1913613/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1351875/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1580699/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:849138/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:3158319/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:2577835/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1998273/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1883677/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:225046/68‑1 (MQ=255) cATGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:2305788/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1021748/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1039396/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:2432136/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:730440/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:576454/68‑1 (MQ=255) cATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:37445/68‑1 (MQ=255) aTGAATGTTCGTACGTTTGTGCTGGTAAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1070986/67‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTgaga < 1:1937277/67‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:4584/69‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:1147478/69‑1 (MQ=255) aTGAATGTCCGAACGTTTGTGCCGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:1810472/69‑1 (MQ=255) tGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg > 1:875938/1‑68 (MQ=255) gAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGa < 1:2506770/69‑1 (MQ=255) gAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGa < 1:3404751/69‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAATATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:403367/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:552252/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:2554983/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:1591984/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:1499896/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:2154108/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga < 1:2958388/62‑1 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:2165478/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:467215/1‑62 (MQ=255) aaTGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTga > 1:332086/1‑62 (MQ=255) ccGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATg < 1:2572001/61‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:725226/68‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:1233350/68‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:1207252/68‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:3349451/68‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:469507/68‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:3144950/68‑1 (MQ=255) cGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTcc < 1:2974132/68‑1 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGCCCGGTGAGATGGACATTCCACTCaa > 1:967740/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:687274/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:1146348/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:292635/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:231651/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:2265749/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:357235/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:1102386/1‑68 (MQ=255) tttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:2213003/1‑68 (MQ=255) cttGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCaa > 1:626047/2‑68 (MQ=255) tgtgCTGGTGAAAGATAAAAAAACATTCTTTTCATCTGCGACCGGTgaga < 1:2254268/50‑1 (MQ=255) | GCTAACCCCGAACTGACCGCCCGCGCAGCGTTTAGCATGAATGTCCGAACGTTTGTGCTGGTGAAAGATAAAAAAACATTCTGTTCATCTGCGACCGGTGAGATGGACATTCCACTCAA > minE/1402392‑1402510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |