| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,402,727 | C→T | 42.6% | G190G (GGC→GGT) | rtn → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,402,727 | 0 | C | T | 42.6% | 27.9 / 59.9 | 82 | G190G (GGC→GGT) | rtn | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (24/23); new base T (16/19); total (40/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.47e-01 | |||||||||||
CCTGACGCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGACATGC > minE/1402661‑1402788 | ccTGACGCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCaa < 1:1737173/69‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTGATACTg < 1:2414664/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2588546/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:3289016/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2341530/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2310168/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:50444/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2154716/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2114021/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:637443/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:1903112/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:1712441/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:822539/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:1637860/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:3063781/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:3033443/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2668567/68‑1 (MQ=255) gCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTAAGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:1577903/68‑1 (MQ=255) ccTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:27094/67‑1 (MQ=255) ctctTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCt > 1:531938/1‑69 (MQ=255) ctctTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCt > 1:747063/1‑69 (MQ=255) ctctTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCt > 1:27067/1‑69 (MQ=255) ctctTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCt > 1:2973538/1‑69 (MQ=255) ctctTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCt > 1:2866845/1‑69 (MQ=255) ctctTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCt > 1:1816047/1‑69 (MQ=255) ttttATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAAt > 1:2098915/1‑54 (MQ=255) ttttATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAAt < 1:3208549/54‑1 (MQ=255) ttttATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAAt > 1:2346778/1‑54 (MQ=255) cACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTttctt > 1:2085123/1‑66 (MQ=255) cACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCAc > 1:1456828/1‑69 (MQ=255) cACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCAc > 1:210059/1‑69 (MQ=255) cACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCAc > 1:372953/1‑69 (MQ=255) aaGATTACGATGGCCTAGCCCTCATTATTGGTAATACTg < 1:2714015/39‑1 (MQ=255) ttACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACg > 1:2602791/1‑68 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:3182905/69‑1 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:3067882/69‑1 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:3132583/69‑1 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:2288237/69‑1 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:2031900/69‑1 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:1964311/69‑1 (MQ=255) aCGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:1390713/69‑1 (MQ=255) cGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:457057/68‑1 (MQ=255) cGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTa < 1:3020707/68‑1 (MQ=255) aTGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAAAGTTa < 1:3322039/66‑1 (MQ=255) tGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGa < 1:983614/69‑1 (MQ=255) tGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGa < 1:2190158/69‑1 (MQ=255) tGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGa < 1:273852/69‑1 (MQ=255) tGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGa < 1:3238939/69‑1 (MQ=255) tGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGa < 1:699929/69‑1 (MQ=255) ggCGTCGCCCTCATTATTGGCAATACTGCGCTATc > 1:2710500/1‑35 (MQ=255) ggCGTCGCCCTCATTATTGGCAATACTGCGCTATc > 1:717013/1‑35 (MQ=255) ggCGTCGCCCTCATTATTGGCAATACTGCGCTATc > 1:702622/1‑35 (MQ=255) ggCGTCGCCCTCATTATTGGCAATACTGCGCTATc > 1:3257908/1‑35 (MQ=255) ggCGTCGCCCTCATTATTGGCAATACTGCGCTATc > 1:3430527/1‑35 (MQ=255) ggCGTCGCCCTCATTATTGGCAATACTGCGCTATc > 1:2266095/1‑35 (MQ=255) gCGTCTCCCTCATTATTGGCAATACTGCGCTATCTACCTTttc > 1:2135101/1‑43 (MQ=255) gCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGaa > 1:2779450/1‑68 (MQ=255) gCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGaa > 1:550083/1‑68 (MQ=255) gCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGaa > 1:2758325/1‑68 (MQ=255) gCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGaa > 1:3238862/1‑68 (MQ=255) ccTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGa < 1:864435/69‑1 (MQ=255) ccTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGa < 1:851745/69‑1 (MQ=255) ccTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGa < 1:1264674/69‑1 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTttcttc > 1:1906022/1‑38 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:1230902/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:977744/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:971163/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:1357429/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:1370872/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:1724887/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:3002453/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:321969/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:2174028/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:2268439/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:36489/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:2474510/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:3334198/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:2565808/1‑40 (MQ=255) cTCATTATTGGTAATACTGCGCTATCTACCTTTTCTTCAc > 1:268062/1‑40 (MQ=255) tCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTa < 1:1007828/62‑1 (MQ=255) tattGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGACATGc < 1:1293500/68‑1 (MQ=255) attGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGACATGc < 1:659367/67‑1 (MQ=255) | CCTGACGCCTTCACTCTTTTATAGTTCACGGCAGGAAGATTACGATGGCGTCGCCCTCATTATTGGCAATACTGCGCTATCTACCTTTTCTTCACGTTTGATGAACGTTAACGAATTAACCGACATGC > minE/1402661‑1402788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |