| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 111,412 | T→C | 67.5% | Y326Y (TAT→TAC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 111,412 | 0 | T | C | 67.5% | 85.7 / 49.7 | 98 | Y326Y (TAT→TAC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base T (26/6); new base C (51/15); total (77/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01 | |||||||||||
ACCAGCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAGT > minE/111361‑111474 | aCCAGCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCt > 1:1792795/1‑69 (MQ=255) gCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGAc > 1:2789144/1‑59 (MQ=255) gCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGAc > 1:314933/1‑59 (MQ=255) gCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGAc > 1:2452468/1‑59 (MQ=255) gCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGAc > 1:633184/1‑59 (MQ=255) gCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGAc > 1:2147643/1‑59 (MQ=255) gCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATccgc > 1:987879/1‑69 (MQ=255) gCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATccg > 1:1489329/1‑68 (MQ=255) cAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGa > 1:1042530/1‑69 (MQ=255) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2142918/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:216010/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2303038/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2344317/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2516076/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2640525/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2689738/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1945321/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2804196/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2849082/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:2876532/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:3093270/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:3206223/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:3330374/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:3396628/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:499819/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:499833/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:541180/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:777198/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:903686/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1272239/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1250446/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1461760/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1166726/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1592620/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1726485/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1723704/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1628750/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1418211/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1857167/1‑53 (MQ=39) aTCTGAATTTGCTGAAAATGACGCTAACGATCACGCGACACCGCTGATccgcc > 1:3343988/1‑53 (MQ=255) aTCAGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATccgcc > 1:1067427/1‑53 (MQ=38) tCTGAATTTGCTTAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:3080181/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTc > 1:2124581/1‑55 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTc > 1:1005172/1‑55 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:2795407/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:3019993/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1492581/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:3404619/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:3427940/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:419704/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1206784/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:523217/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1034164/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1569698/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1951016/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:2024854/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:2190225/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1595704/1‑56 (MQ=255) tCTGAATTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:1595344/1‑56 (MQ=255) tCTGAACTTGCTGAAAATGACGCTTACGTTCACGCGACTCCGCTGATCCGCCGTCt > 1:439421/1‑56 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGttt > 1:1816227/1‑68 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:2279219/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:186325/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:932010/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:568585/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:452078/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:2657185/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:3358481/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:3306392/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:2653236/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:100006/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:1306074/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:1414695/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:2938308/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:2455312/1‑69 (MQ=255) cTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTg > 1:3093991/1‑69 (MQ=255) tGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTgg > 1:197980/1‑69 (MQ=255) acgCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:2417101/69‑1 (MQ=255) acgCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:438861/69‑1 (MQ=255) acgCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:1124453/69‑1 (MQ=255) cgCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:725842/68‑1 (MQ=255) cgCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:1096290/68‑1 (MQ=255) cgCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:607029/68‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCTAGTTTGGCGTTAACCTGGCGAAAGt < 1:892905/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2776497/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2637621/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2718542/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:738473/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:97160/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2833460/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:501805/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2136773/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:1379067/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:3431275/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2272263/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:1249758/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:2302917/66‑1 (MQ=255) ttACGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGCGTTAACCTGGCGAAAGt < 1:1367250/66‑1 (MQ=255) tGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAg < 1:3131798/62‑1 (MQ=255) | ACCAGCTGCGAAAGCGGAAGGCAAATCTGAATTTGCTGAAAACGACGCTTATGTTCACGCGACTCCGCTGATCCGCCGTCTGGCACGCGAGTTTGGTGTTAACCTTGCGAAAGT > minE/111361‑111474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |