| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,403,233 | A→C | 42.9% | D359A (GAC→GCC) | rtn → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,403,233 | 0 | A | C | 42.9% | 1.7 / 49.5 | 49 | D359A (GAC→GCC) | rtn | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (26/2); new base C (9/12); total (35/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01 | |||||||||||
GCGATGCCGCAGAATTAGAAAAAGTGCTGCCGGTAGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGCA > minE/1403167‑1403294 | gcgATGCCGCAGAATTAGAAAAAGTGCTGCCGGTAGGCGTCAAATTTGGTATTAACATTGCGCCGGAcc < 1:3073422/69‑1 (MQ=255) cgATGCCGCAGATTTAGAAAAAGTGCTGCCGGTAGGCGTCAAATTTGGTATTAACATTGCGCCGGAcc < 1:3043012/68‑1 (MQ=255) aaTTAGAAAAAGTACTGCCGGTAGGCGTCAAATTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCg < 1:3341182/69‑1 (MQ=255) aaTTAGAAAAAGTACTGCCGGTAGGCGTCAAATTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCg < 1:1377287/69‑1 (MQ=255) tAGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGata > 1:2893130/1‑66 (MQ=255) tAGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGata > 1:2114244/1‑66 (MQ=255) tAGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGata > 1:584142/1‑66 (MQ=255) aGGCGTCAAATTTGGTATTAAGATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATcc > 1:2294002/1‑68 (MQ=255) aGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATcc > 1:2297790/1‑68 (MQ=255) aGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATcc > 1:3212364/1‑68 (MQ=255) aGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATcc > 1:2782946/1‑68 (MQ=255) gTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGaaa > 1:3193555/1‑69 (MQ=255) gTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGaaa > 1:3369020/1‑69 (MQ=255) gTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGaaa > 1:1796584/1‑69 (MQ=255) cAAATTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAG‑AGATATCCAGAAACTg < 1:1474096/69‑1 (MQ=255) aaaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:430281/69‑1 (MQ=255) aaaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:1891949/69‑1 (MQ=255) aaaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:1875353/69‑1 (MQ=255) aaaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:2755300/69‑1 (MQ=255) aaaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:3287216/69‑1 (MQ=255) aaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:1924519/68‑1 (MQ=255) aaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:2808129/68‑1 (MQ=255) aaTTTGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:13799/68‑1 (MQ=255) ttGGCATTAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTg < 1:841413/65‑1 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTccc > 1:2273725/1‑68 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:3348648/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:2410533/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:2221960/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:2196782/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:813402/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:1399758/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCTTTAAAGCGGATATCCAGAAACTGCTCACTTCCCt > 1:86670/1‑69 (MQ=255) tAACATTGCGCCAGCCCACTTGCACAGCGAAAGCATTAAAGCGGaaat > 1:2885354/1‑48 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAGAGCTTTAAAGCAGATATCCAGa > 1:1358038/1‑52 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGa > 1:3236588/1‑52 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGa > 1:2478745/1‑52 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGa > 1:2643961/1‑52 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCt > 1:1358902/1‑68 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTg > 1:1069973/1‑69 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTg > 1:2210302/1‑69 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTg > 1:626870/1‑69 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTg > 1:1411727/1‑69 (MQ=255) aaCATTGCGCCGGACCATCTGCACAGCGAAAGCTATAAAGCAGATATCCAGa > 1:3247264/1‑52 (MQ=255) aaCATTGCGCCGGACCATCTGAACAGCGAAAGCTTTAAAGCAGATATcc > 1:2636501/1‑49 (MQ=255) gcgcCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGACACTGCTCACTTCCCTGCCCGc > 1:1085938/1‑68 (MQ=255) gcgcCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGca > 1:2156103/1‑69 (MQ=255) gcgcCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGca > 1:1987304/1‑69 (MQ=255) gcgcCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGca > 1:1931908/1‑69 (MQ=255) gcgcCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGc > 1:2940371/1‑68 (MQ=255) | GCGATGCCGCAGAATTAGAAAAAGTGCTGCCGGTAGGCGTCAAATTTGGTATTAACATTGCGCCGGACCATCTGCACAGCGAAAGCTTTAAAGCAGATATCCAGAAACTGCTCACTTCCCTGCCCGCA > minE/1403167‑1403294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |