| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,406,881 | A→G | 100% | L59L (TTA→TTG) | yejE → | predicted oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,406,881 | 0 | A | G | 75.2% | 41.5 / 8.9 | 36 | L59L (TTA→TTG) | yejE | predicted oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/9); new base G (0/27); total (0/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTG > minE/1406815‑1406949 | ttCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTAtt < 1:1813507/69‑1 (MQ=255) cTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAAc < 1:2736051/69‑1 (MQ=255) ttATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAAc < 1:2176484/68‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1241920/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:905139/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:853573/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:659918/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:605984/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:419519/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:408433/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:3345775/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:2913328/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:2744843/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:262744/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:2563216/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:222812/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1998399/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1868461/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1355639/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1509133/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1836214/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1741943/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1514401/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1615768/67‑1 (MQ=255) cGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:1611391/67‑1 (MQ=255) gCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:2042907/66‑1 (MQ=255) ccAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:674837/65‑1 (MQ=255) cGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:2349009/61‑1 (MQ=255) ccGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:831977/54‑1 (MQ=255) tGCGTTATGACGGCAGTTGGTATTTCCCGTTGTTGAAAAACTAc < 1:2798273/44‑1 (MQ=255) aGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCt < 1:1659718/69‑1 (MQ=255) aGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCt < 1:1877835/69‑1 (MQ=255) ggTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAg < 1:1267217/62‑1 (MQ=255) ggTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAg < 1:1182989/62‑1 (MQ=255) ggTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGAtt < 1:2491420/69‑1 (MQ=255) aTTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATc < 1:264945/69‑1 (MQ=255) aTTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGt > 1:2650434/1‑68 (MQ=255) aTTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTg > 1:3093302/1‑69 (MQ=255) | TTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTG > minE/1406815‑1406949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |