| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,408,514 | G→A | 52.3% | P261P (CCG→CCA) | yejF → | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,408,514 | 0 | G | A | 52.3% | ‑1.0 / 49.2 | 58 | P261P (CCG→CCA) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (9/18); new base A (14/16); total (23/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
TACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAG > minE/1408457‑1408577 | tacgctaTTTGCATCACCCACTCATCCTTACACACAAAATCTACTCAACAGTGAACCGTCAGGCGATcc < 1:3109436/69‑1 (MQ=255)tacgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATcc < 1:805437/69‑1 (MQ=255) acgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCTTCAGGCGATcc < 1:297250/68‑1 (MQ=255) acgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCa > 1:2437753/1‑69 (MQ=255) cACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAg < 1:3286970/69‑1 (MQ=255) ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGaa < 1:805914/68‑1 (MQ=255) ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGaa < 1:749067/68‑1 (MQ=255) cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:170363/1‑69 (MQ=255) cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:1769735/1‑69 (MQ=255) cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:686253/1‑69 (MQ=255) cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:3338646/1‑69 (MQ=255) cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:682365/1‑69 (MQ=255) cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:2867504/1‑69 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTTAACGTTGCTGGATGTTg < 1:3023576/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:3207870/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:2933077/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:3130351/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:2791023/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:519928/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:2110158/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1893609/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1739016/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1720089/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:152035/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1308846/69‑1 (MQ=255) tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1249627/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:395955/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3405314/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:335550/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3350410/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:460882/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3348959/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3293252/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:918743/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:1378990/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2731508/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3140664/69‑1 (MQ=255) aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2491926/69‑1 (MQ=255) cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:1872699/68‑1 (MQ=255) cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2955465/68‑1 (MQ=255) cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2800901/68‑1 (MQ=255) cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:494897/68‑1 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATTTTGAACAGc > 1:2548203/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:446865/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:1088672/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:833307/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2226866/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:535454/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2305741/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2577156/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2759635/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2906270/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:3034219/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:3126153/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:3148283/1‑68 (MQ=255) cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCCAGATGTTGAACAGc > 1:1125806/1‑68 (MQ=255) aGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGc > 1:1671635/1‑67 (MQ=255) gAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAg > 1:3171000/1‑69 (MQ=255) | TACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAG > minE/1408457‑1408577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |