| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 112,771 | C→T | 49.4% | T40T (ACC→ACT) | lpd → | lipoamide dehydrogenase, E3 component is part of three enzyme complexes |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 112,771 | 0 | C | T | 49.4% | ‑1.0 / 82.4 | 91 | T40T (ACC→ACT) | lpd | lipoamide dehydrogenase, E3 component is part of three enzyme complexes |
| Reads supporting (aligned to +/- strand): ref base C (8/38); new base T (21/24); total (29/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01 | |||||||||||
TTACTCCGCTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAC > minE/112705‑112825 | ttACTCCGCTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCt > 1:2913931/1‑69 (MQ=255) cTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGgtg < 1:1074487/69‑1 (MQ=255) cTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGgtg < 1:2246141/69‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:402209/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:2995835/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:1763138/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:3182417/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:3201090/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:2566429/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:2494019/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:3369606/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:3383014/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:1421856/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:1370268/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:47800/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:2184582/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:221199/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:837665/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:87303/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:976387/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:2676717/68‑1 (MQ=255) gTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACTCTTGGCGGTGTTTGCCTg < 1:2280294/68‑1 (MQ=255) gcgcTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACg > 1:3374567/1‑69 (MQ=255) gcgcTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACg > 1:615175/1‑69 (MQ=255) gcgcTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACg > 1:932083/1‑69 (MQ=255) gcgcTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACg > 1:1795149/1‑69 (MQ=255) gcgcTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACg > 1:3006039/1‑69 (MQ=255) gcTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGG‑GGTGTTTGCCTGAACGTc < 1:994791/68‑1 (MQ=255) cTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTc < 1:2975667/68‑1 (MQ=255) cTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTc < 1:2774312/68‑1 (MQ=255) cTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTc < 1:58511/68‑1 (MQ=255) cTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTc < 1:708610/68‑1 (MQ=255) cTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTc < 1:1188690/68‑1 (MQ=255) ttAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTg < 1:282334/68‑1 (MQ=255) ttAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTg < 1:1008/68‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:2616085/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:2433055/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:3345027/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:3361391/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:990183/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:2374852/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:1009044/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:120419/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:124427/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:1280195/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:1441560/48‑1 (MQ=255) gAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTg < 1:1585841/48‑1 (MQ=255) aaaCCGTAATCGTAGAACGTTACAACACCCTTGGCGgtg < 1:1945178/39‑1 (MQ=255) ccGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:2779518/69‑1 (MQ=255) ccGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:2294979/69‑1 (MQ=255) ccGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:1468446/69‑1 (MQ=255) ccGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:3340998/69‑1 (MQ=255) ccGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:68562/69‑1 (MQ=255) ccGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:3303422/69‑1 (MQ=255) cGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAg < 1:1919603/68‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:3419750/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:2267553/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:1277794/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:1737557/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:1092743/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:90406/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:1050176/69‑1 (MQ=255) tAATCGTAGAACGTTACAACACCCTTGGCGGGGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCAc < 1:2304037/69‑1 (MQ=255) gTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCActgctg > 1:3314606/1‑69 (MQ=255) gTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCActgctg > 1:1678214/1‑69 (MQ=255) gAACGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAc < 1:1181288/69‑1 (MQ=255) gAACGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAc < 1:470574/69‑1 (MQ=255) gAACGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAc < 1:1378201/69‑1 (MQ=255) gAACGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAc < 1:2417595/69‑1 (MQ=255) gAACGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAc < 1:3233575/69‑1 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTGTAAAGCACTGCTGCa > 1:2871414/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:719159/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:2100717/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:1035856/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:2096812/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:1055852/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:915367/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:903171/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:2007908/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:1892940/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:720700/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:339450/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:2554678/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:3168470/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:628865/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:1690209/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:602344/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:593452/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:2587996/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:471536/1‑67 (MQ=255) aaCGTTACAACACTCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCa > 1:1676660/1‑67 (MQ=255) | TTACTCCGCTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACCCTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCAC > minE/112705‑112825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |