| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,408,853 | G→A | 56.8% | S374S (TCG→TCA) | yejF → | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,408,853 | 0 | G | A | 56.8% | 16.3 / 57.3 | 74 | S374S (TCG→TCA) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (25/7); new base A (20/22); total (45/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.09e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
ACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCTT > minE/1408805‑1408919 | aCCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGt < 1:75821/69‑1 (MQ=255) cGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAg > 1:825932/1‑69 (MQ=255) cGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAg > 1:1273598/1‑69 (MQ=255) cGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAg > 1:1739434/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:587727/1‑67 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:1427221/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:2044217/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:2300406/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:2348701/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:1832839/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:2508829/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:2772031/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:3170432/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:1410465/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:3257367/1‑69 (MQ=255) cAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGaa > 1:965942/1‑69 (MQ=255) tggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCt < 1:883064/69‑1 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGatta > 1:1334037/1‑56 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:3223536/1‑62 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:1062076/1‑62 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:3092460/1‑62 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:3273285/1‑62 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:2474975/1‑62 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:3375834/1‑62 (MQ=255) ggtATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAgg > 1:1946895/1‑62 (MQ=255) tCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACggg > 1:1405789/1‑68 (MQ=255) tCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACggg > 1:2759525/1‑68 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGc > 1:879497/1‑40 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:1619824/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:3348195/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:544618/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:1437909/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:3118334/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:653661/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:1184858/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:2822653/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:789781/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:1666785/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:1986311/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:2125/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:215192/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:2307618/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:2400483/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCaa > 1:2489099/1‑41 (MQ=255) gATCCAAACTCCTCACTCAACCCACGACTCAACGTTTTGCa > 1:1437325/1‑41 (MQ=255) gATCCAAACTCCTCAATCAACCCACGACTCAACGTTTTGCaa > 1:2965504/1‑41 (MQ=38) gATCCAAACTCCTAACTCAACCCACGACTCAACGTTTTGCaa > 1:2861282/1‑41 (MQ=255) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:692188/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:831908/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:599503/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:111358/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1218723/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1003120/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:562449/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:2026477/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1318819/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1136403/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1399196/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:3223607/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:3211707/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:3125032/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1909784/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:1512883/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:3016709/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:3331712/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:2842717/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:2590679/37‑2 (MQ=39) aaaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:2556835/37‑2 (MQ=39) aaCTCCTCACTCAACCCACGACTCAACGTTTTGCaa < 1:2849399/36‑2 (MQ=39) cGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCtt < 1:822693/68‑1 (MQ=255) cGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCtt < 1:1860072/68‑1 (MQ=255) cGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCtt < 1:592083/68‑1 (MQ=255) cGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCtt < 1:2199124/68‑1 (MQ=255) cGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCtt < 1:2070539/68‑1 (MQ=255) | ACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAAACTCCTCGCTCAACCCACGACTCAACGTTTTGCAGATTATTGAGGAAGGCTTACGGGTTCACCAGCCGACGCTT > minE/1408805‑1408919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |