| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,410,533 | A→C | 19.4% | L220L (CTT→CTG) | bcr ← | bicyclomycin/multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,410,533 | 0 | A | C | 19.4% | 57.6 / 10.5 | 36 | L220L (CTT→CTG) | bcr | bicyclomycin/multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (9/20); new base C (4/3); total (13/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01 | |||||||||||
TTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGTTACC > minE/1410467‑1410583 | ttCAATGTAGACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAg < 1:165861/69‑1 (MQ=255)ttCAATGTAGACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAg < 1:2694811/69‑1 (MQ=255) tCAATGTAGACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAg < 1:2084130/68‑1 (MQ=255) ataAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTa > 1:122269/1‑69 (MQ=255) ataAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTa > 1:334708/1‑69 (MQ=255) ataAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTa > 1:1376948/1‑69 (MQ=255) ataAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTa > 1:259921/1‑69 (MQ=255) ataAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTa > 1:2564801/1‑69 (MQ=255) taAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGGAAGCATGTAg < 1:614709/69‑1 (MQ=255) taAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGc > 1:1690492/1‑63 (MQ=255) taAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAg < 1:1437483/69‑1 (MQ=255) aacaaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAg < 1:218531/67‑1 (MQ=255) aaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAGCATATAACTCAg > 1:3105916/1‑69 (MQ=255) aaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAGCATATAACTCAg > 1:3055277/1‑69 (MQ=255) aaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAGCATATAACTCAg > 1:52741/1‑69 (MQ=255) aaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCGCTGGCCAGCATATAACTCAg > 1:1292392/1‑69 (MQ=255) aaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAg < 1:240879/69‑1 (MQ=255) aaacGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAg < 1:1774957/69‑1 (MQ=255) cGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGtt < 1:237069/69‑1 (MQ=255) cGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGtt < 1:866160/69‑1 (MQ=255) aggCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGtt < 1:2067801/68‑1 (MQ=255) gcTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGtt > 1:3348511/1‑65 (MQ=255) gcTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGtt > 1:3264040/1‑65 (MQ=255) gcTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGtt > 1:314877/1‑65 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACTTTTATGGCGGAACAGCGCCGCAAAGt < 1:2645173/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:2938910/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:291539/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:2854869/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:1086822/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:3401870/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:440767/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:2003366/68‑1 (MQ=255) ggCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGt < 1:1395617/68‑1 (MQ=255) aaaGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGTTAcc < 1:843184/69‑1 (MQ=255) aaGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGTTAcc < 1:3269000/68‑1 (MQ=255) gCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGTAACAGCGCCGCAAAGt < 1:1118999/62‑1 (MQ=255) | TTCAATATAAACAAACGGTCCGGCGCTTAAGAATGAGAACATCCCGGCAAAGCTGAAACCACTGGCAAGCATGTAGCTCAGGACACGTTTATGGCGGAACAGCGCCGCAAAGTTACC > minE/1410467‑1410583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |