| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,417,268 | T→G | 22.8% | A487A (GCT→GCG) | yejM → | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,417,268 | 0 | T | G | 22.8% | 187.0 / 62.4 | 127 | A487A (GCT→GCG) | yejM | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base T (52/46); new base G (27/2); total (79/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
CCACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCT > minE/1417202‑1417331 | ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct < 1:1085954/69‑1 (MQ=255)ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct < 1:1758894/69‑1 (MQ=255)ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct < 1:627319/69‑1 (MQ=255)ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct < 1:2414390/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:1065495/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:2035085/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:2103992/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:231660/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:2366171/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:1622499/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:2470841/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:2488478/69‑1 (MQ=255) cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt < 1:3278428/69‑1 (MQ=255) tGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATa > 1:1065854/1‑69 (MQ=255) ggTGCCATTAGTGATTCACTGGCCAGGCACCCCGGCGCAGCGTATTAATGCGCTGACTGATCATACCg < 1:801570/68‑1 (MQ=255) gCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCt > 1:896104/1‑69 (MQ=255) gCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCt > 1:2942133/1‑69 (MQ=255) gCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCt > 1:2349240/1‑69 (MQ=255) tctGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:570603/67‑1 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCtgatga > 1:433899/1‑68 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCtgatg > 1:3062285/1‑67 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:944200/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:213132/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:80991/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:598787/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:3425316/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:2974719/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:3009941/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:3120695/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:1686274/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:158417/1‑69 (MQ=255) aGTGATTCACTGGCCAGGCACGCCGGCACAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:1755416/1‑69 (MQ=255) aTTCACTGGCCAGGCAGGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:2141742/1‑65 (MQ=255) aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:253339/1‑65 (MQ=255) aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:555421/1‑65 (MQ=255) aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:1652682/1‑65 (MQ=255) aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:3392039/1‑65 (MQ=255) aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac > 1:1171289/1‑65 (MQ=255) aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACAGATCTGATgac > 1:3155422/1‑65 (MQ=255) tCACTGGCCAGGCACGCCGTCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:1135766/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:1454941/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:1316895/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:3236348/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:3235310/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:2334970/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:1356121/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:1241030/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:611996/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac < 1:2265288/63‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGc > 1:735773/1‑68 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:736390/69‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:1845909/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:1847387/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:855771/69‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:1921884/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:3259448/69‑1 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:1180761/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:773489/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:367897/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:1316768/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:1595440/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt > 1:2399827/1‑69 (MQ=255) tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:2113232/69‑1 (MQ=255) cACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:1525128/68‑1 (MQ=255) cACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt < 1:1672630/68‑1 (MQ=255) gCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGc < 1:2587790/63‑1 (MQ=255) gCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGcc < 1:1404433/69‑1 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa > 1:1049596/1‑69 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa < 1:2000115/69‑1 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa < 1:796700/69‑1 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa > 1:1824291/1‑69 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa > 1:32876/1‑69 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa > 1:2441657/1‑69 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa < 1:3355230/69‑1 (MQ=255) ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa < 1:1532313/69‑1 (MQ=255) caggCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa < 1:708126/67‑1 (MQ=255) cAGCGTATTAATGCTCTGACTGATCATACCGTTCTGATGACGACGCTGATGCAACGCCTGCTACATGTc < 1:268521/69‑1 (MQ=255) gTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGca < 1:2429521/69‑1 (MQ=255) gTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGca < 1:1218875/69‑1 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:698357/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:1515857/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:186068/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:188124/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:290908/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:2763979/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:263002/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:638870/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:2106003/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:2227875/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:132160/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:1843417/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:429030/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:370250/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCAGCTACATGTc > 1:2008924/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGATCTGAAGACGACGCTGATGCAACGCCTGCTACATGTc > 1:2737055/1‑63 (MQ=255) aTTAATGCGCTGACTGATCATACCGACCTGATGACGACGCTGATGCAACGCCTGCTACATGTc > 1:2605221/1‑63 (MQ=255) aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt > 1:3339245/1‑68 (MQ=255) aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt > 1:1814431/1‑68 (MQ=255) aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt > 1:1895239/1‑68 (MQ=255) aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATCCAACGCCTGc > 1:1288267/1‑51 (MQ=255) | CCACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCT > minE/1417202‑1417331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |