| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 113,344 | C→T | 18.6% | N231N (AAC→AAT) | lpd → | lipoamide dehydrogenase, E3 component is part of three enzyme complexes |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 113,344 | 0 | C | T | 18.6% | 107.4 / 13.5 | 69 | N231N (AAC→AAT) | lpd | lipoamide dehydrogenase, E3 component is part of three enzyme complexes |
| Reads supporting (aligned to +/- strand): ref base C (48/8); new base T (5/8); total (53/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGAC > minE/113292‑113403 | cTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAAcc < 1:1251862/68‑1 (MQ=255) aGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTAc > 1:2980249/1‑69 (MQ=255) aGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTAc > 1:2670490/1‑69 (MQ=255) gACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTAcc < 1:725020/69‑1 (MQ=255) gACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTAcc < 1:1975201/69‑1 (MQ=255) tttAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:2206612/2‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCt < 1:1436795/41‑1 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTAccg > 1:1834962/1‑64 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:486261/1‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:2310481/1‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:848910/1‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:1909035/1‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:376101/1‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:2900557/1‑68 (MQ=255) gTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACAAAAGTTACCGACGt > 1:886006/1‑68 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:2487515/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:476139/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:2585660/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:1925869/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:196043/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:861812/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:1285394/1‑66 (MQ=255) cTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:3009382/1‑66 (MQ=255) cTTCAACAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGc > 1:875025/1‑66 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGaa > 1:2463671/1‑68 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGaa > 1:2702375/1‑68 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGaa > 1:2986121/1‑68 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGaa > 1:1723736/1‑68 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGaa > 1:2132664/1‑68 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGa > 1:2063176/1‑67 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGa > 1:1500379/1‑67 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTCGAAGCGaa > 1:2468111/1‑68 (MQ=255) ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTAGAAGCGaa > 1:875666/1‑68 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:3085880/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:105359/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:1274571/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:3050142/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:1706712/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:2406753/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:239705/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaagaag > 1:1889795/1‑69 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaag < 1:1436987/66‑1 (MQ=255) ccAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCCTTGAAGCGAaaga > 1:1324204/1‑67 (MQ=255) cAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAaag < 1:1308768/65‑1 (MQ=255) tATCAGCAAGAAATTCAACCTGATGCTGGAAACAAAAGTTACCGCCGTTGAAGCGAaag < 1:1221560/59‑1 (MQ=255) tCAGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGt > 1:2331188/1‑46 (MQ=255) tCAGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:378339/1‑69 (MQ=255) tCAGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:380132/1‑69 (MQ=255) tCAGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:205067/1‑69 (MQ=255) tCAGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAtt > 1:2955649/1‑68 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:2650171/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:1357588/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:880406/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:2014545/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:355935/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:2984255/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:540673/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAttt > 1:756616/1‑69 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAtt > 1:2011696/1‑68 (MQ=255) tCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCAtt > 1:470651/1‑68 (MQ=255) aGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:232314/68‑1 (MQ=255) aGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:2154506/68‑1 (MQ=255) aGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:2202652/68‑1 (MQ=255) aGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:3192202/68‑1 (MQ=255) aGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:3071529/68‑1 (MQ=255) aGCAAGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:3058338/68‑1 (MQ=255) aGAAATTCAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:2568111/64‑1 (MQ=255) gAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGAc < 1:1626657/69‑1 (MQ=255) cAATCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTa < 1:947668/57‑1 (MQ=255) | CTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGAC > minE/113292‑113403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |