| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,422,660 | C→T | 100% | V91V (GTG→GTA) | ccmE ← | periplasmic heme chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,422,660 | 0 | C | T | 87.7% | 23.4 / ‑3.0 | 16 | V91V (GTG→GTA) | ccmE | periplasmic heme chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (0/14); total (0/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTA > minE/1422610‑1422680 | ccctgccctTCACGTAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:1751638/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGATACATCTACTGAACCTTCAGCGTCg < 1:2491080/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:1851456/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:1961037/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:2218006/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:228284/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:2289579/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:2998757/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:413267/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:673013/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:77076/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:806019/69‑1 (MQ=255)ccctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:945656/69‑1 (MQ=255) cctgccctTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCTACTGAACCTTCAGCGTCg < 1:642713/68‑1 (MQ=255) cACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTa < 1:156732/61‑1 (MQ=255) gCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTa < 1:3380683/39‑1 (MQ=255) | CCCTGCCCTTCACGGAACAGATCCGGCAAAATGCCTTCGTAAGAGACATCCACTGAGCCTTCAGCATCGTA > minE/1422610‑1422680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |