| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,426,109 | A→T | 21.0% | D54E (GAT→GAA) | napB ← | nitrate reductase, small, cytochrome C550 subunit, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,426,109 | 0 | A | T | 21.0% | 137.3 / 28.7 | 76 | D54E (GAT→GAA) | napB | nitrate reductase, small, cytochrome C550 subunit, periplasmic |
| Reads supporting (aligned to +/- strand): ref base A (4/56); new base T (8/8); total (12/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGC > minE/1426047‑1426163 | tGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGc < 1:3139154/69‑1 (MQ=255)tGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGc < 1:1094534/69‑1 (MQ=255)tGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGc < 1:559757/69‑1 (MQ=255)tGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGc < 1:7716/69‑1 (MQ=255) gATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGc < 1:2060730/68‑1 (MQ=255) aaCCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCtt > 1:1930579/1‑69 (MQ=255) aaCCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCtt > 1:2550075/1‑69 (MQ=255) aaCCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCtt > 1:1565985/1‑69 (MQ=255) ttCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTgg < 1:2679513/69‑1 (MQ=255) ttCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTgg < 1:686482/69‑1 (MQ=255) ttCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTgg < 1:359329/69‑1 (MQ=255) tCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCTGCATCCGATCCTGCTCTTTTGGc < 1:2609431/69‑1 (MQ=255) cAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCa > 1:282029/1‑69 (MQ=255) cAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCa > 1:1679293/1‑69 (MQ=255) cAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCa > 1:1877244/1‑69 (MQ=255) cAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCa > 1:1046817/1‑69 (MQ=255) gCTATGCGGGATCATTGGCGGCTGCTTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:3308014/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:496626/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:3246939/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:2886524/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:1004985/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:376764/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:2492840/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:2901939/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:2367886/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:2168954/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:3185773/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:1043251/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:527909/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:1912939/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:1371907/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:1413034/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:643493/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:1524752/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:163121/69‑1 (MQ=255) gCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCAGCATCCGATCCTGCTCTTTTGGCATCCg < 1:728794/69‑1 (MQ=255) cTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:2890437/68‑1 (MQ=255) aTGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAAt < 1:2136994/69‑1 (MQ=255) aTGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAAt < 1:1484911/69‑1 (MQ=255) aTGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAAt < 1:2680607/69‑1 (MQ=255) cGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGc < 1:1617740/69‑1 (MQ=255) cGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGc < 1:642696/69‑1 (MQ=255) gggATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCg < 1:428330/62‑1 (MQ=255) tCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCtt < 1:1648214/69‑1 (MQ=255) tCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCtt < 1:385350/69‑1 (MQ=255) tCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCtt < 1:2894429/69‑1 (MQ=255) tCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCtt < 1:1168810/69‑1 (MQ=255) tCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCta < 1:1584927/69‑2 (MQ=255) aTTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCtt < 1:2012251/67‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:2541644/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:545303/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:394981/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:320239/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:811213/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:2821284/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:1436990/43‑1 (MQ=255) tGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:2335458/43‑1 (MQ=255) ggcggcTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCtgc > 1:873543/1‑68 (MQ=255) ggcggcTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCtgc > 1:657285/1‑68 (MQ=255) ggcggcTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCtgc > 1:1861260/1‑68 (MQ=255) ggcggcTGATTCACATAGTTCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCtgc > 1:24996/1‑68 (MQ=255) ggcTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTc > 1:2976752/1‑69 (MQ=255) gATTCACATAGTTCAGCGGCATCCGATCCTGctct < 1:1902733/35‑1 (MQ=255) tCACATAGTTCAGCGGCATCCGATCCTGCTTTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGa < 1:2681268/67‑1 (MQ=255) tCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGa < 1:25232/67‑1 (MQ=255) tCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGa < 1:54516/67‑1 (MQ=255) tCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGa < 1:1726362/67‑1 (MQ=255) tCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGa < 1:2727984/67‑1 (MQ=255) tCATCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:1959971/68‑1 (MQ=255) tCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:2087407/68‑1 (MQ=255) tCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:1904078/68‑1 (MQ=255) tCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:3426727/68‑1 (MQ=255) tCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:1349728/68‑1 (MQ=255) tCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:108656/68‑1 (MQ=255) tCAGCGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCACCTTCCTGCGTCCCGGATACTTCCGGc < 1:1616757/68‑1 (MQ=255) cGGCATCCGTTCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGCGTCCCGGATACTTCCGGc < 1:1958147/64‑1 (MQ=255) | TGATAACCTTCAACGCTATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGC > minE/1426047‑1426163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |