| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,431,974 | T→C | 100% | intergenic (+29/+686) | eco → / ← mqo | ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,431,974 | 0 | T | C | 92.1% | 28.5 / ‑3.9 | 24 | intergenic (+29/+686) | eco/mqo | ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (0/22); total (0/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACG > minE/1431920‑1432010 | taaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2106152/68‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2655637/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:989638/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:88120/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:648615/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:618424/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:583626/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:56144/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:3346937/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:3061655/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2969428/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2557863/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2473020/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2368966/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:2002342/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:1869313/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:179769/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:1649403/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:1525332/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:1495857/69‑2 (MQ=12)aaaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:1407314/69‑2 (MQ=12) aaTTGACAACGCGGTTGTCCGCTAAACTGCTGTGAAGTGCTGCAACCTGTAGGCCGAATAAGGCGGtt < 1:3358739/68‑2 (MQ=12) cgGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCg < 1:1279549/69‑1 (MQ=255) tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACg < 1:2770481/68‑1 (MQ=255) | AAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACG > minE/1431920‑1432010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |