| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,433,227 | T→C | 38.1% | L360L (CTA→CTG) | mqo ← | malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,433,227 | 0 | T | C | 38.1% | 37.1 / 48.8 | 76 | L360L (CTA→CTG) | mqo | malate dehydrogenase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base T (28/19); new base C (16/13); total (44/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAC > minE/1433170‑1433292 | aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:1551264/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:2095028/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:2284516/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:19870/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:2418889/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:1788731/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:1621809/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:812980/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:155792/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:3388460/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:1488436/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:378131/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:1158938/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:117150/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:1372145/1‑68 (MQ=255)aTTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCcaca > 1:93039/1‑68 (MQ=255) catcatCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGaa < 1:1475709/69‑1 (MQ=255) catcatCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGaa < 1:3166572/69‑1 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:824609/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:2184293/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:2338106/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:894268/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:195407/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:567214/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:415430/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:490371/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:1409279/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:1514857/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:1441139/68‑3 (MQ=255) cGGCATCACGTTAGAGGTGGTGGTGGAACTCATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:429851/68‑3 (MQ=255) agGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCa < 1:2503205/68‑1 (MQ=255) agGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCa < 1:2763331/68‑1 (MQ=255) agGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCa < 1:1074684/68‑1 (MQ=255) agGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCa < 1:660577/68‑1 (MQ=255) agGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCa < 1:1473788/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:2080293/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:2006934/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:777308/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:1914602/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:1258431/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:1446664/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:1707543/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:2987222/68‑1 (MQ=255) ggtggtggtGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCaa < 1:3007740/68‑1 (MQ=255) gtggtggtgGAACTCATTAGATCCCTCAATGAACCGTTTTTGAGGAATTTGGTTGAGAATGTGGCAAAt > 1:2848542/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGTAAAt > 1:1513510/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:853172/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:772644/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:577587/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:1459096/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:2403129/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:2489773/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:1744135/1‑69 (MQ=255) gtggtggtgGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAAt > 1:2832202/1‑69 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:407241/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:1849097/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:3032728/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:2898328/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:3355456/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:1567940/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:3233967/1‑68 (MQ=255) gtggtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATgg > 1:822255/1‑68 (MQ=255) gtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCa > 1:2755994/1‑69 (MQ=255) gtggAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCa > 1:1608314/1‑69 (MQ=255) aaCTAATCAGATCCCACAATGAACCGTTTTTGAGGAAtttag < 1:2952085/42‑3 (MQ=38) aCTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTTGTTGAGAAGGTGGCAAATGGCCCAAACAg < 1:897819/69‑1 (MQ=255) aCTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAg > 1:1101436/1‑69 (MQ=255) aCTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAg < 1:3349037/69‑1 (MQ=255) aCTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAg < 1:3179364/69‑1 (MQ=255) cATTAGATCCCTCAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAc > 1:1965585/1‑69 (MQ=255) cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAACCAGCAc > 1:97800/1‑69 (MQ=255) cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAc > 1:2727975/1‑69 (MQ=255) cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAc > 1:2031334/1‑69 (MQ=255) cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAc > 1:1374488/1‑69 (MQ=255) cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAc > 1:1283397/1‑69 (MQ=255) cATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAc > 1:959421/1‑69 (MQ=255) | ATTATCCAGCCCGACGTGCATCATCGGCATCACGTTAGAGGTGGTGGTGGAACTCATTAGATCCCACAATGAACCGTTTTTGAGGAATTTGGTTGAGAAGGTGGCAAATGGCCCAAACAGCAC > minE/1433170‑1433292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |